Comparison of parasitological, serological, and molecular tests for visceral leishmaniasis in HIV-infected patients: a cross-sectional delayed-type study

dc.creatorCota, Gláucia Fernandes
dc.creatorSousa, Marcos Roberto de
dc.creatorNogueira, Betânia Mara de Freitas
dc.creatorGomes, Luciana Inácia
dc.creatorOliveira, Edward José de
dc.creatorAssis, Tália Santana Machado de
dc.creatorMendonça, Andréa Laender Pessoa de
dc.creatorPinto, Bruna Fernandes
dc.creatorSaliba, Juliana Wilke
dc.creatorRabello, Ana Lúcia Teles
dc.date2018-12-03T16:34:27Z
dc.date2018-12-03T16:34:27Z
dc.date2013
dc.date.accessioned2023-09-27T00:13:13Z
dc.date.available2023-09-27T00:13:13Z
dc.identifierCOTA, Gláucia Fernandes et al. Comparison of parasitological, serological, and molecular tests for visceral leishmaniasis in HIV-infected patients: a cross-sectional delayed-type study. American Journal of Tropical Medicine and Hygiene, v. 89, n. 3, p. 570-577, 2013.
dc.identifier0002-9637
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30366
dc.identifier10.4269/ajtmh.13-0239
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898642
dc.descriptionThe aim of this study was to evaluate the accuracy of invasive and non-invasive tests for diagnosis of visceral leishmaniasis (VL) in a large series of human immunodeficiency virus (HIV)-infected patients. In this delayed-type cross-sectional study, 113 HIV-infected symptomatic patients were evaluated by an adjudication committee after clinical follow-up to establish the presence or absence of VL as the target condition (reference test). The index tests were recombinant K39 antigen-based immunochromatographic test (rK39), indirect fluorescent antibody test (IFAT), prototype kit of direct agglutination test (DAT-LPC), and real-time polymerase chain reaction (qPCR) in peripheral blood. Compared with parasitological test and adjudication committee diagnosis or latent class model analyses, IFAT and rk39 dipstick test presented the lowest sensitivity. DAT-LPC exhibited good overall performance, and there was no statistical difference between DAT-LPC and qPCR diagnosis accuracy. Real-time PCR emerges as a less invasive alternative to parasitological examination for confirmation of cases not identified by DAT.
dc.description2100-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society of Tropical Medicine and Hygiene
dc.rightsrestricted access
dc.subjectLeishmaniose visceral
dc.subjectVisceral leishmaniasis
dc.titleComparison of parasitological, serological, and molecular tests for visceral leishmaniasis in HIV-infected patients: a cross-sectional delayed-type study
dc.typeArticle


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