Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment

dc.creatorSarmento, Sylvia Kahwage
dc.creatorGuerra, Caroline Rezende
dc.creatorMalta, Fábio Correia
dc.creatorCoutinho, Ricardo
dc.creatorMiagostovich, Marize Pereira
dc.creatorFumian, Tulio Machado
dc.date2021-02-21T13:33:04Z
dc.date2021-02-21T13:33:04Z
dc.date2020
dc.date.accessioned2023-09-27T00:13:09Z
dc.date.available2023-09-27T00:13:09Z
dc.identifierSARMENTO, Sylvia Kahwage et al. Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment. Marine Pollution Bulletin, v. 157, p. 1-9, June 2020.
dc.identifier0025-326X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/46121
dc.identifier10.1016/j.marpolbul.2020.111315
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898634
dc.descriptionNoroviruses are the most common cause of gastroenteritis outbreaks in humans and bivalve shellfish consumption is a recognized route of infection. Our aim was to detect and characterize norovirus in bivalves from a coastal city of Brazil. Nucleic acid was extracted from the bivalve's digestive tissue concentrates using magnetic beads. From March 2018 to June 2019, 77 samples were screened using quantitative RT-PCR. Noroviruses were detected in 41.5%, with the GII being the most prevalent (37.7%). The highest viral load was 3.5 × 106 and 2.5 × 105 GC/g in oysters and mussels, respectively. PMA-treatment demonstrated that a large fraction of the detected norovirus corresponded to non-infectious particles. Genetic characterization showed the circulation of the GII.2[P16] and GII.4[P4] genotypes. Norovirus detection in bivalves reflects the anthropogenic impact on marine environment and serves as an early warning for the food-borne disease outbreaks resulting from the consumption of contaminated molluscs.
dc.description2023
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectNorovirus
dc.subjectOstras
dc.subjectMexilhões
dc.subjectTratamento PMA
dc.subjectReserva marinha
dc.subjectRio de Janeiro
dc.subjectNorovirus
dc.subjectOysters
dc.subjectMussels
dc.subjectPMA treatment
dc.subjectMarine reserve
dc.subjectRio de Janeiro
dc.titleHuman norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment
dc.typeArticle


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