| dc.creator | Masid-de-Brito, Daniela | |
| dc.creator | Queto, Túlio | |
| dc.creator | Gaspar-Elsas, Maria Ignez C. | |
| dc.creator | Xavier-Elsas, Pedro Paulo | |
| dc.date | 2015-03-04T14:51:10Z | |
| dc.date | 2015-03-04T14:51:10Z | |
| dc.date | 2014 | |
| dc.date.accessioned | 2023-09-27T00:13:07Z | |
| dc.date.available | 2023-09-27T00:13:07Z | |
| dc.identifier | MASID-DE-BRITO, Daniela. et al. Roles of 5-lipoxygenase and cysteinyl-leukotriene type 1 receptors in the hematological response to allergen challenge and its prevention by diethylcarbamazine in a murine model of asthma. Mediat. inflamm., New York, 2014. | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/9616 | |
| dc.identifier | 10.1155/2014/403970 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8898626 | |
| dc.description | Diethylcarbamazine (DEC), which blocks leukotriene production, abolishes the challenge-induced increase in eosinopoiesis
in bone-marrow from ovalbumin- (OVA-) sensitized mice, suggesting that 5-lipoxygenase (5-LO) products contribute to the
hematological responses in experimental asthma models. We explored the relationship between 5-LO, central and peripheral
eosinophilia, and effectiveness of DEC, using PAS or BALB/cmice and 5-LO-deficient mutants. We quantified eosinophil numbers
in freshly harvested or cultured bone-marrow, peritoneal lavage fluid, and spleen, with or without administration of leukotriene
generation inhibitors (DEC and MK886) and cisteinyl-leukotriene type I receptor antagonist (montelukast). The increase in
eosinophil numbers in bone-marrow, observed in sensitized/challenged wild-type mice, was abolished by MK886 and DEC
pretreatment. InALOXmutants, by contrast, therewas no increase inbone-marroweosinophil counts, nor ineosinophil production
in culture, in response to sensitization/challenge. In sensitized/challengedALOX mice, challenge-inducedmigration of eosinophils
to the peritoneal cavity was significantly reduced relative to the wild-type PAS controls. DEC was ineffective in ALOX mice, as
expected from a mechanism of action dependent on 5-LO. In BALB/c mice, challenge significantly increased spleen eosinophil
numbers andDECtreatment prevented this increase.Overall, 5-LOappears as indispensable to the systemichematological response
to allergen challenge, as well as to the effectiveness of DEC | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Hindawi Publishing Corporation | |
| dc.rights | open access | |
| dc.subject | Araquidonato 5-Lipoxigenase | |
| dc.subject | Dietilcarbamazina | |
| dc.subject | Medula Óssea | |
| dc.subject | Eosinófilos | |
| dc.subject | Aloxano | |
| dc.title | Roles of 5-lipoxygenase and cysteinyl-leukotriene type 1 receptors in the hematological response to allergen challenge and its prevention by diethylcarbamazine in a murine model of asthma | |
| dc.type | Article | |
