Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom

dc.creatorTeixera, Leda Fabiélen
dc.creatorCarvalho, Letícia Helena de
dc.creatorCastro, Onássis Boeri de
dc.creatorBastos, Jéssica Silva Félix
dc.creatorNéry, Neriane Monteiro
dc.creatorOliveira, George Azevedo
dc.creatorKayano, Anderson Makoto
dc.creatorSoares, Andreimar Martins
dc.creatorZuliani, Juliana Pavan
dc.date2019-06-10T19:58:07Z
dc.date2019-06-10T19:58:07Z
dc.date2017
dc.date.accessioned2023-09-27T00:13:03Z
dc.date.available2023-09-27T00:13:03Z
dc.identifierTEIXEIRA, Leda Fabiélen et al. Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom. Toxicon, v. 141, p. 55-64, 2017.
dc.identifier0041-0101
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33448
dc.identifier10.1016/j.toxicon.2017.11.007
dc.identifier1879-3150
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898616
dc.descriptionO artigo encontra-se disponível para download no site do Editor.
dc.descriptionThe present work aimed to isolate a basic phospholipase A2 (PLA2) from Bothrops diporus snake venom (BdV), evaluate and compare the myotoxic and oedema-inducing activities, as well as the systemic effects caused by both the isolated PLA2 and BdV on Swiss mice. A Lys-49 PLA2 (BdipTX-I) was obtained through two chromatographic steps: an ion-exchange and a reverse phase. The local (oedema and myotoxicity) and systemic (hepatic and renal functions) effects were then assessed for BdipTX-I and BdV. Results showed that the oedema-inducing activity was significant in all tested doses (5 and 20 mg/paw) for both BdipTX-I and BdV. Myotoxicity was evaluated by the increase of serum CK, CK-MB and LDH, and results showed that BdV effect is more prominent than BdipTX-I effect. The systemic effects were evaluated by determining specific laboratory markers: AST, ALT, GGT, ALP, urea, creatinine, protein and calcium. BdipTX-I and BdV were able to induce renal changes in the experimental model, leading to proteinuria (induced both by BdipTX-I and by BdV) and uremia (induced only by BdV). Thus, it is concluded that the systemic effects of BdV and BdipTX-I occur differently.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectPhospholipase A2
dc.subjectBothrops diporus
dc.subjectOedema
dc.subjectMyotoxicity
dc.subjectSystemic effect
dc.titleLocal and systemic effects of BdipTX-I, a Lys-49 phospholipase A2 isolated from Bothrops diporus snake venom
dc.typeArticle


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