Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx‑qPCR

dc.creatorMacena, Lorena da Graça Pedrosa de
dc.creatorPereira, Joseane Simone de Oliveira
dc.creatorSilva, Jansen Couto da
dc.creatorFerreira, Fernando César
dc.creatorMaranhão, Adriana Gonçalves
dc.creatorLanzarini, Natália Maria
dc.creatorMiagostovic, Marize Pereira
dc.date2022-07-31T13:16:11Z
dc.date2022-07-31T13:16:11Z
dc.date2022
dc.date.accessioned2023-09-27T00:12:55Z
dc.date.available2023-09-27T00:12:55Z
dc.identifierMACENA, Lorena da Graça Pedrosa de et al. Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx‑qPCR. Brazilian Journal of Microbiology, on line, p. 1 - 7, May 2022.
dc.identifier1678-4405
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/54090
dc.identifier10.1007/s42770-022-00775-5
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898591
dc.descriptionMolecular methodologies providing data on viral concentration and infectivity have been successfully used in environmental virology, supporting quantitative risk assessment studies. The present study aimed to assess human mastadenovirus (HAdV) intact particles using a derivative of propidium monoazide associated with qPCR (PMAxx-qPCR) in aquatic matrices. Initially, different concentrations of PMAxx were evaluated to establish an optimal protocol for treating different naturally contaminated matrices, using 10 min incubation in the dark at 200 rpm at room temperature and 15 min of photoactivation in the PMA-Lite™ LED photolysis device. There was no significant reduction in the quantification of infectious HAdV with increasing concentration of PMAxx used (20 μM, 50 μM, and 100 μM), except for sewage samples. In this matrix, a reduction of 5.01 log of genomic copies (GC)/L was observed from the concentration of 50 μM and revealed 100% HAdV particles with damaged capsids. On the other hand, the mean reduction of 0.51 log in stool samples using the same concentration mentioned above demonstrated 83% of damaged particles eliminated in the stool. Following, 50 μM PMAxx-qPCR protocol revealed a log reduction of 0.91, 0.67, and 1.05 in other samples of raw sewage, brackish, and seawater where HAdV concentration reached 1.47 × 104, 6.81 × 102, and 2.33 × 102 GC/L, respectively. Fifty micrometers of PMAxx protocol helped screen intact viruses from different matrices, including sea and brackish water.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedade Brasileira de Microbiologia
dc.rightsopen access
dc.subjectMonoazida de propídio
dc.subjectPMAxx-qPCR
dc.subjectEsgoto bruto
dc.subjectÀgua salobra
dc.subjectÁgua do mar
dc.subjectHAdV
dc.subjectPropidium monoazide
dc.subjectPMAxx-qPCR
dc.subjectHAdV
dc.subjectRaw sewage
dc.subjectBrackish water
dc.subjectSeawater
dc.titleQuantification of infectious Human mastadenovirus in environmental matrices using PMAxx‑qPCR
dc.typeArticle


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