Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.

dc.creatorMenezes, Juliana Perrone Bezerra de
dc.creatorAlmeida, Taís Fontoura de
dc.creatorPetersen, Antonio Luis de Oliveira Almeida
dc.creatorGuedes, Carlos Eduardo Sampaio
dc.creatorMota, M. S.
dc.creatorLima, José Geraldo Bomfim
dc.creatorPalma, Luana Carneiro
dc.creatorBuck, Gregory A.
dc.creatorKrieger, Marco Aurélio
dc.creatorProbst, Christian M.
dc.creatorVeras, Patrícia Sampaio Tavares
dc.date2014-05-29T19:29:39Z
dc.date2014-05-29T19:29:39Z
dc.date2013
dc.date.accessioned2023-09-27T00:12:54Z
dc.date.available2023-09-27T00:12:54Z
dc.identifierMENEZES, J. P. B. et al. Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major. Microbes and Infection, v. 15, n. 8-9, p. 579-591, 2013.
dc.identifier1769-714X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7725
dc.identifierdx.doi.org/10.1016/j.micinf.2013.04.005
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898588
dc.descriptionCBA macrophages effectively control Leishmania major infection, yet are permissive to Leishmania amazonensis. Employing a transcriptomic approach, we previously showed the up-regulation of the genes involved in the classical pathway of macrophage activation in resistant mice. However, microarray analyses do not evaluate changes in gene expression that occur after translation. To circumvent this analytical limitation, we employed a proteomics approach to increase our understanding of the modulations that occur during infection and identify novel targets for the control of Leishmania infection. To identify proteins whose expression changes in CBA macrophages infected with L. major or L. amazonensis, protein extracts were obtained and digested and the peptides were characterized using multi-dimensional liquid chromatography coupled with tandem mass spectrometry analyses. A total of 162 proteins were selected as potentially modulated. Using biological network analyses, these proteins were classified as primarily involved in cellular metabolism and grouped into cellular development biological networks. This study is the first to use a proteomics approach to describe the protein modulations involved in cellular metabolism during the initial events of Leishmania-macrophage interaction. Based on these findings, we hypothesize that these differentially expressed proteins likely play a pivotal role in determining the course of infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Masson SAS
dc.rightsopen access
dc.subjectLeishmania amazonensis
dc.subjectLeishmania major
dc.subjectProteomics
dc.subjectMacrophages
dc.subjectInterações Hospedeiro-Patógeno
dc.subjectLeishmania major/imunologia
dc.subjectLeishmania mexicana/imunologia
dc.subjectMacrófagos/química
dc.subjectMacrófagos/parasitologia
dc.subjectProteoma/análise
dc.subjectAnimais
dc.subjectCromatografia Líquida
dc.subjectFeminino
dc.subjectLeishmania major/patogenicidade
dc.subjectMacrófagos/imunologia
dc.subjectMasculino
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos CBA
dc.subjectEspectrometria de Massas em Tandem
dc.titleProteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major.
dc.typeArticle


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