Ticks as potential vectors of Mycobacterium leprae: Use of tick cell lines to culture the bacilli and generate transgenic strains

dc.creatorFerreira, Jéssica da Silva
dc.creatorOliveira, Diego Augusto
dc.creatorSantos, João Pedro Souza
dc.creatorRibeiro, Carla Carolina Dias Uzedo
dc.creatorBaêta, Bruna A.
dc.creatorTeixeira, Rafaella Câmara
dc.creatorNeumann, Arthur da Silva
dc.creatorRosa, Patricia Sammarco
dc.creatorPessolani, Maria Cristina Vidal
dc.creatorMoraes, Milton Ozório
dc.creatorBechara, Gervásio Henrique
dc.creatorOliveira, Pedro L. de
dc.creatorSorgine, Marcos Henrique Ferreira
dc.creatorSuffys, Philip Noel
dc.creatorFontes, Amanda Nogueira Brum
dc.creatorBell-Sakyi, Lesley
dc.creatorFonseca, Adivaldo H.
dc.creatorLara, Flavio Alves
dc.date2019-07-02T17:12:15Z
dc.date2019-07-02T17:12:15Z
dc.date2018
dc.date.accessioned2023-09-27T00:12:50Z
dc.date.available2023-09-27T00:12:50Z
dc.identifierFERREIRA, Jéssica da Silva et al. Ticks as potential vectors of Mycobacterium leprae: Use of tick cell lines to culture the bacilli and generate transgenic strains. PLoS Neglected Tropical Diseases, v. 12, n. 12, p. 1-25, Dec. 2018.
dc.identifier1935-2727
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33781
dc.identifier10.1371/journal.pntd.0007001
dc.identifier1935-2735
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898578
dc.descriptionLeprosy is an infectious disease caused by Mycobacterium leprae and frequently resulting in irreversible deformities and disabilities. Ticks play an important role in infectious disease transmission due to their low host specificity, worldwide distribution, and the biological ability to support transovarial transmission of a wide spectrum of pathogens, including viruses, bacteria and protozoa. To investigate a possible role for ticks as vectors of leprosy, we assessed transovarial transmission of M. leprae in artificially-fed adult female Amblyomma sculptum ticks, and infection and growth of M. leprae in tick cell lines. Our results revealed M. leprae RNA and antigens persisting in the midgut and present in the ovaries of adult female A. sculptum at least 2 days after oral infection, and present in their progeny (eggs and larvae), which demonstrates the occurrence of transovarial transmission of this pathogen. Infected tick larvae were able to inoculate viable bacilli during blood-feeding on a rabbit. Moreover, following inoculation with M. leprae, the Ixodes scapularis embryo-derived tick cell line IDE8 supported a detectable increase in the number of bacilli for at least 20 days, presenting a doubling time of approximately 12 days. As far as we know, this is the first in vitro cellular system able to promote growth of M. leprae. Finally, we successfully transformed a clinical M. leprae isolate by inserting the reporter plasmid pCHERRY3; transformed bacteria infected and grew in IDE8 cells over a 2-month period. Taken together, our data not only support the hypothesis that ticks may have the potential to act as a reservoir and/or vector of leprosy, but also suggest the feasibility of technological development of tick cell lines as a tool for large-scale production of M. leprae bacteria, as well as describing for the first time a method for their transformation.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectCarrapatos
dc.subjectVetores
dc.subjectMycobacterium leprae
dc.subjectBacilos
dc.subjectCepas trangênicas
dc.subjectLinhas de células de carrapatos
dc.subjectMycobacterium leprae
dc.subjectVectors
dc.subjectTicks
dc.subjectBacilli
dc.subjectTransgenic strains
dc.subjectTick cell lines
dc.titleTicks as potential vectors of Mycobacterium leprae: Use of tick cell lines to culture the bacilli and generate transgenic strains
dc.typeArticle


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