Serological and virological survey of hepatitis E virus (HEV) in animal reservoirs from Uruguay reveals elevated prevalences and a very close phylogenetic relationship between swine and human strains

dc.creatorMirazo, Santiago
dc.creatorGardinali, Noemí R.
dc.creatorCecilia, D'Albora
dc.creatorVerger, Lorenzo
dc.creatorOttonelli, Florencia
dc.creatorRamos, Natalia
dc.creatorCastro, Gustavo
dc.creatorPinto, Marcelo A.
dc.creatorRé, Viviana
dc.creatorPisano, Belén
dc.creatorLozano, Alejandra
dc.creatorOliveira, Jaqueline Mendes de
dc.creatorArbiza, Juan
dc.date2018-05-24T16:22:31Z
dc.date2018-05-24T16:22:31Z
dc.date2018
dc.date.accessioned2023-09-27T00:12:41Z
dc.date.available2023-09-27T00:12:41Z
dc.identifierMIRAZO, Santiago; et al. Serological and virological survey of hepatitis E virus (HEV) in animal reservoirs from Uruguay reveals elevated prevalences and a very close phylogenetic relationship between swine and human strains. Veterinanry Microbiology, v.213, p.21-27, 2018.
dc.identifier0378-1135
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26637
dc.identifier10.1016/j.vetmic.2017.11.013
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898563
dc.descriptionHepatitis E virus (HEV) infection is an issue of public health concern in high-income and non-endemic countries. Increasing evidence supports the hypothesis of a zoonotic route as the main mode of infection in this epidemiological setting, since the transmission of genotypes HEV-3 and HEV-4 from reservoirs to humans has been demonstrated. In America, studies have confirmed the circulation of HEV in pig herds but the zoonotic role of wild boars has never been evaluated. Uruguay has a high burden of HEV- associated acute hepatitis, and a close phylogenetic relationship was observed among human HEV-3 strains and European isolates detected in swine. However in this context, swine herds have never been surveyed. Herein is reported a survey of HEV in swine herds, pigs at slaughter-house and free-living wild boar populations. Two-hundred and twenty sera and 150 liver tissue samples from domestic pigs, and 140 sera from wild boars were tested for HEV by ELISA and PCR-based approaches. All tested swine farms resulted seropositive with an overall rate of 46.8%. In turn, 22.1% of the wild boars had anti-HEV antibodies. HEV RNA was detected in 16.6% and 9.3% of liver samples from slaughter-age pigs and adult wild boars sera, respectively. Three strains from domestic pig were also amplified by nested-PCR approaches. By contrast, none of the positive samples obtained from wild boars could be confirmed by nested-PCR. Phylogenetic analysis revealed a very high nucleotide identity among swine strains and sequences obtained from humans in Uruguay. Results showed that HEV is widely distributed among swine herds in Uruguay. Additionally, this study evidences for the first time in the American continent that wild boar populations are a reservoir for HEV, though its zoonotic role remains to be elucidated. Altogether, data presented here suggest a high zoonotic risk of HEV transmission from swine to humans.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectVírus da Hepatite E
dc.subjectSus scrofa
dc.subjectPesquisa
dc.subjectUruguai
dc.subjectHepatitis E virus
dc.subjectDomestic pig
dc.subjectWild boars
dc.subjectSurvey
dc.subjectUruguay
dc.titleSerological and virological survey of hepatitis E virus (HEV) in animal reservoirs from Uruguay reveals elevated prevalences and a very close phylogenetic relationship between swine and human strains
dc.typeArticle


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