Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?

dc.creatorLima, Monique da Rocha Queiroz
dc.creatorLima, Raquel Curtinhas de
dc.creatorAzevedo, Elzinandes Leal de
dc.creatorSantos, Flavia Barreto dos
dc.date2021-08-03T13:51:19Z
dc.date2021-08-03T13:51:19Z
dc.date2021
dc.date.accessioned2023-09-27T00:12:40Z
dc.date.available2023-09-27T00:12:40Z
dc.identifierLIMA, Monique da Rocha Queiroz et al. Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?. Diagnostics, v. 11, 819, 11 p, Apr. 2021.
dc.identifier2075-4418
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/48485
dc.identifier10.3390/diagnostics11050819
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898561
dc.descriptionIn Brazil, chikungunya emerged in 2014, and by 2016, co-circulated with other arbovirosis, such as dengue and zika. ELISAs (Enzyme-Linked Immunosorbent Assays) are the most widely used approach for arboviruses diagnosis. However, some limitations include antibody cross reactivities when viruses belong to the same genus, and sensitivity variations in distinct epidemiological scenarios. As chikungunya virus (CHIKV) is an alphavirus, no serological cross reactivity with dengue virus (DENV) should be observed. Here, we evaluated a routinely used chikungunya commercial IgM (Immunoglobulin M) ELISA test (Anti-Chikungunya IgM ELISA, Euroimmun) to assess its performance in confirming chikungunya in a dengue endemic area. Samples (n = 340) representative of all four DENV serotypes, healthy individuals and controls were tested. The Anti-CHIKV IgM ELISA test had a sensitivity of 100% and a specificity of 25.3% due to the cross reactivities observed with dengue. In dengue acute cases, the chikungunya test showed an overall cross-reactivity of 31.6%, with a higher cross-reactivity with DENV-4. In dengue IgM positive cases, the assay showed a cross-reactivity of 46.7%. Serological diagnosis may be challenging and, despite the results observed here, more evaluations shall be performed. Because distinct arboviruses co-circulate in Brazil, reliable diagnostic tools are essential for disease surveillance and patient management.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectDiagnóstico de chikungunia
dc.subjectAnti-chikungunia igM ELISA
dc.subjectReatividade cruzada da dengue
dc.subjectBrasil
dc.subjectChikungunia diagnosis
dc.subjectAnti-chikungunia igM ELISA
dc.subjectDengue cross-reactivity
dc.subjectBrazil
dc.titleAnalysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?
dc.typeArticle


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