Comparison of five methods for the determination of lethal dose in acute toxicity studies

dc.creatorPaumgartten, Francisco José Roma
dc.creatorPresgrave, Octavio Augusto França
dc.creatorMenezes, Marco Antônio Carneiro
dc.creatorFingola, Fernando Faria
dc.creatorFreitas, João Carlos Borges Rolim de
dc.creatorCarvalho, Rosangela Ribeiro de
dc.creatorCunha, Fernando de Queiroz
dc.date2015-07-14T00:47:04Z
dc.date2015-07-14T00:47:04Z
dc.date1989
dc.date.accessioned2023-09-27T00:12:34Z
dc.date.available2023-09-27T00:12:34Z
dc.identifierPAUMGARTTEN, F. J. R. et al. Comparison of five methods for the determination of lethal dose in acute toxicity studies. Braz. J. Med. Biol. Res. São Paulo, v. 22, n. 8, p. 987-991, 1989.
dc.identifier0100-879X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/11351
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898545
dc.descriptionThe aim of the present study was to compare the reliability of LD50 determination using the traditional Litchfield and Wilcoxon method with that obtained by four alternative tests requiring smaller numbers of animals, for the purpose of classifying chemicals according to their acute toxicity. Acute lethal dose determinations were carried out in mice for oral and intraperitoneal administration of hexachlorophene, lidocaine, methanol, phenobarbital and physostigmine. The Molinengo method proved not to be as reliable as suggested by its author. Determination of LD50 using the Thompson and Weil method or, alternatively, the maximal non-lethal dose and the approximate lethal dose permitted the classification of the chemicals in essentially the same order. The approximate lethal dose method, in particular, seems to be a very suitable alternative method to the classical LD50 test since it requires only about 6 animals, provides enough information to order chemicals according to their toxicities, and provides useful information for planning subsequent repeated-dose studies.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.rightsopen access
dc.subjectAlternativas aos Testes com Animais
dc.subjectHexaclorofeno
dc.subjectDose Letal Mediana
dc.subjectLidocaína
dc.subjectCamundongos
dc.subjectFenobarbital
dc.subjectFisostigmina
dc.subjectAnimal Testing Alternatives
dc.subjectHexachlorophene
dc.subjectLethal Dose 50
dc.subjectLidocaine
dc.subjectMice
dc.subjectPhenobarbital
dc.subjectPhysostigmine
dc.subjectAlternativas aos Testes com Animais
dc.subjectHexaclorofeno
dc.subjectDose Letal Mediana
dc.subjectLidocaína
dc.subjectCamundongos
dc.subjectFenobarbital
dc.subjectFisostigmina
dc.titleComparison of five methods for the determination of lethal dose in acute toxicity studies
dc.typeArticle


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