Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms

dc.creatorCorrêa, José R.
dc.creatorAtella, Georgia C.
dc.creatorVargas, Camila
dc.creatorSoares, Maurilio J.
dc.date2020-01-20T19:05:31Z
dc.date2020-01-20T19:05:31Z
dc.date2007
dc.date.accessioned2023-09-27T00:12:18Z
dc.date.available2023-09-27T00:12:18Z
dc.identifierCORRÊA, José R. et al. Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 7, p. 871-876, Nov. 2007.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/39328
dc.identifier10.1590/s0074-02762007005000117
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898499
dc.descriptionUptake of transferrin by epimastigote forms of the protozoan Trypanosoma cruzi occurs mainly through a cytostome/ cytopharynx, via uncoated endocytic vesicles that bud off from the bottom of the cytopharynx. We have here examined whether detergent-resistant membrane (DRM) domains might be involved in this process. Purified whole cell membrane fractions were assayed for cholesterol levels and used in dot blot analyses. Detergent-resistant membrane markers (cholera B toxin and anti-flotillin-1 antibody) presented positive reaction by dot blots in cholesterol-rich/ protein-poor membrane sub-fractions. The positive dot blot fraction was submitted to lipid composition analysis, showing composition similar to that of raft fractions described for other eukaryotic cells. Immunofluorescence assays allowed the localization of punctual positive signal for flotillin-1, matching the precise cytostome/ cytopharynx location. These data were confirmed by immunofluorescence assays with the co-localization of flotillin-1 and the transferrin uptake site. Our data suggest that DRM domains occur and are integrated at the cytostome/ cytopharynx of T. cruzi epimastigotes, being the main route for transferrin uptake.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectEndocitose
dc.subjectMembrana resistente a detergente
dc.subjectTrypanosoma cruzi
dc.subjectUltraestrutura
dc.subjectFormas de epimastigote
dc.subjectCytopharynx
dc.subjectDetergent-resistant membrane
dc.subjectEndocytosis
dc.subjectTrypanosoma cruzi
dc.subjectUltrastructure
dc.subjectEpimastigote forms
dc.titleTransferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi epimastigote forms
dc.typeArticle


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