Immunoenzymatic assay for the diagnosis of American tegumentary leishmaniasis using soluble and membrane-enriched fractions from infectious Leishmania (Viannia) braziliensis

dc.creatorCataldo, Jamyra Iglesias
dc.creatorMello, Fernanda Carvalho de Queiroz
dc.creatorMouta-Confort, Eliame
dc.creatorMadeira, Maria de Fátima
dc.creatorSchubach, Armando de Oliveira
dc.creatorGenestra, Marcelo da Silva
dc.creatorRibeiro, Flávia Coelho
dc.creatorMoreira-Venâncio, Célia de Fátima
dc.creatorPassos, Sônia Regina Lambert
dc.date2019-03-15T12:06:55Z
dc.date2019-03-15T12:06:55Z
dc.date2010
dc.date.accessioned2023-09-27T00:12:06Z
dc.date.available2023-09-27T00:12:06Z
dc.identifierCATALDO, Jamyra Iglesias et al. Immunoenzymatic assay for the diagnosis of American tegumentary leishmaniasis using soluble and membrane-enriched fractions from infectious Leishmania (Viannia) braziliensis. Journal of Clinical Laboratory Analysis, v. 24, p. 289–294, 2010.
dc.identifier0887-8013
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32101
dc.identifier10.1002/jcla.20402
dc.identifier1098-2825
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898465
dc.descriptionThe diagnosis of American tegumentary leishmaniasis (ATL) is based on the visualization or isolation of the parasite, which is a time-consuming and poorly sensitive method. In this study, we evaluated the accuracy and reliability of ELISA for the diagnosis of ATL using soluble (SF) and membrane-enriched (MF) antigen fractions obtained from an infectious strain of Leishmania (Viannia) braziliensis. A total of 152 serum samples investigated at a referral center in Rio de Janeiro, Brazil, between 2005 and 2007 were studied. Each sample was tested twice with each fraction for the calculation of reliability (intraclass coefficient (ICC)). Cut-off values of 0.22 (SF) and 0.33 (MF) were defined. The use of the fractions resulted in good discrimination between patients, with a large area under the curve (P<0.0001), but no difference was observed between the two fractions (P=0.45). Sensitivity was 89.5% for each fraction, specificity was 89.5% for SF and 93.4% for MF, and the positive likelihood ratio was 8.5 for SF and 13.6 for MF. The ICCs were excellent (SF: 0.96 and MF: 0.90). The antigens tested provided precision and accuracy for the diagnosis of ATL, with SF being recommended due to its lower cost and greater practicality.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsopen access
dc.subjectELISA
dc.subjectLeishmania (Viannia) braziliensis
dc.subjectReliability and validity
dc.subjectValidation studies
dc.subjectDiagnostic techniques and procedures
dc.titleImmunoenzymatic assay for the diagnosis of American tegumentary leishmaniasis using soluble and membrane-enriched fractions from infectious Leishmania (Viannia) braziliensis
dc.typeArticle


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