Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis

dc.creatorCordeiro, Taps Aparecida Reis
dc.creatorMartins, Helen Rodrigues
dc.creatorFranco, Diego Leoni
dc.creatorSantos, Fred Luciano Neves
dc.creatorCeledon, Paola Alejandra Fiorani
dc.creatorCantuaria, Vinicius Lopes
dc.creatorLana, Marta de
dc.creatorReis, Alexandre Barbosa
dc.creatorFerreira, Lucas Franco
dc.date2020-10-28T12:53:03Z
dc.date2020-10-28T12:53:03Z
dc.date2020
dc.date.accessioned2023-09-27T00:11:28Z
dc.date.available2023-09-27T00:11:28Z
dc.identifierCORDEIRO, Taps Aparecida Reis et al. Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis. Biosensors and Bioelectronics, 2020.
dc.identifier0956-5663
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44242
dc.identifier10.1016/j.bios.2020.112573
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898353
dc.descriptionFundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil, (Project: APQ 03097 15).
dc.descriptionIn this work, a dual detection system based on an impedimetric immunosensor was developed for the lust time for the simultaneous detection of anti-Tgpanosoma cruzi and anti-Leishmania infanturn antibodies in human and dog serum samples. The IBMP 8.1 and rLcilA/rLci2B recombinant antigens were inunobilized over the surface of dual screen-printed carbon electrodes (W1 and W2) modified with poly (4-hydroxyphenylacetic acid). Under optimized conditions, the immunosensor recognized specific interactions for anti-T. cruzi antibodies up to a dilution of 1:10,240 and for anti-L. infant-tun up to 1:5120 in canine seams samples. Relative standard deviation (RSD) values of 2.8% for W1 and 3.6% for W2 were obtained for T. cruzi (W1) and L infannun antigen (W2) samples in three different electrodes for 3 days (n = 9). The immunosensor was stored at 4 °C for 8 weeks, with activity retention of 70.2% in W1 and 78.2% in W2. The results using the recombinant proteins revealed that all antigens discriminated between negative and positive samples (p < 0.0001) in both dog and human groups, as well as no cross-reactivity could be detected among sera with other infections. With this approach, immunosensor-based diagnostic tests achieved 100% accuracy, suggesting that the antigens are eligible to enter Phase-II studies.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectChagas
dc.subjectLeishmaniose visceral
dc.subjectDetecção dupla
dc.subjectImunossensor impedimétrico
dc.subjectEletropolimerização
dc.subjectChagas
dc.subjectVisceral leishmaniasis
dc.subjectDual detection
dc.subjectImpedimetric immunosensor
dc.subjectElectropolymerization
dc.titleImpedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
dc.typeArticle


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