Steady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles

dc.creatorCelestino, J. J. H.
dc.creatorLima-Verde, I. B.
dc.creatorBruno, J. B.
dc.creatorMatos, M. H. T.
dc.creatorChaves, R. N.
dc.creatorSaraiva, M. V. A.
dc.creatorSilva, C. M. G.
dc.creatorFaustino, L. R.
dc.creatorRossetto, R.
dc.creatorLopes, C. A. P.
dc.creatorDonato, M. A. M.
dc.creatorPeixoto, C. A.
dc.creatorCampello, C. C.
dc.creatorSilva, J. R. V.
dc.creatorFigueiredo, J. R.
dc.date2019-01-21T13:03:13Z
dc.date2019-01-21T13:03:13Z
dc.date2011
dc.date.accessioned2023-09-27T00:11:12Z
dc.date.available2023-09-27T00:11:12Z
dc.identifierCELESTINO, J. J. H. et al. Steady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles. Molecular and Cellular Endocrinology, v. 338, n. 1, p. 1–9, 16 maio 2011.
dc.identifier1872-8057
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31170
dc.identifier10.1016/j.mce.2011.02.007
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898306
dc.descriptionFaculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil Institute for Research and Technology, Tiradentes University, Aracaju, SE, Brazil Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil Laboratory of Ultrastructure, CPqAM/Fiocruz, Federal University of Pernambuco, Recife, PE, Brazil Biotecnology Nucleus of Sobral (NUBIS), Federal University of Ceara, Sobral, CE, Brazil
dc.descriptionNational Council for Scientific and Technological Development (CNPq), and the Coordination for the Improvement of Higher Education Personnel (CAPES), and the Brazilian Innovation Agency (FINEP) and the Ceara State Foundation for the Support of Scientific and Technological Development (FUNCAP). Juliana Jales de Hollanda Celestino is a recipient of a grant from FUNCAP.
dc.descriptionThis study investigates steady-state level of bone morphogenetic protein-15 (BMP-15) mRNA in caprine follicles, and the effects of BMP-15 on in vitro development of preantral follicles. Ovarian fragments were cultured for one or seven days in Minimal Essential Medium (MEM(+)) with BMP-15 (0, 1, 10, 50, 100 or 200 ng/mL), and further analyzed by histology, transmission electron and fluorescent microscopy. BMP-15 mRNA in secondary follicles was higher than in primordial and primary follicles. After seven days, 10, 50 or 100 ng/mL of BMP-15 maintained the percentage of normal follicles similar to the control (non-cultured), and increased the oocyte and follicle diameters when compared to the control and MEM(+). BMP-15 at 100 ng/mL increased the secondary follicles and maintained their ultrastructural integrity. In conclusion, the BMP-15 mRNAs were detected in all follicular categories. BMP-15 (100 ng/mL) maintained the integrity and promoted the growth of caprine preantral follicles cultured for seven days.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectCaprine
dc.subjectPreantral follicles
dc.subjectBMP-15
dc.subjectActivation
dc.subjectGrowth
dc.subjectAnimais
dc.subjectProteína Morfogenética Óssea 15 / genética
dc.subjectMorfogenética Óssea 15 / metabolismo
dc.subjectProteína Morfogenética Óssea 15 / farmacologia
dc.subjectSobrevivência Celular / efeitos de drogas
dc.subjectFêmea
dc.subjectCaprinos / crescimento e desenvolvimento
dc.subjectMicroscopia Eletrônica, Transmissão
dc.subjectOócitos / efeitos de drogas
dc.subjectOócitos / crescimento e desenvolvimento
dc.subjectFolículo Ovariano / anatomia & histologia
dc.subjectFolículo Ovariano / efeitos de drogas
dc.subjectFolículo Ovariano / crescimento e desenvolvimento
dc.subjectFolículo Ovariano / metabolismo
dc.subjectTécnicas de cultura de tecidos
dc.subjectTranscrição Genética
dc.titleSteady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles
dc.typeArticle


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