Identification and characterization of sex-linked proteins of Schistosoma mansoni

dc.creatorMaldonado Jr, Arnaldo
dc.creatorSimone, Giovanni de
dc.date2020-09-17T13:16:22Z
dc.date2020-09-17T13:16:22Z
dc.date1991
dc.date.accessioned2023-09-27T00:11:04Z
dc.date.available2023-09-27T00:11:04Z
dc.identifierMALDONADO JR., A.; SIMONE, Giovanni de. Identification and characterization of sex-linked proteins of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 1, p. 31-36, Jan./Mar. 1991.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43422
dc.identifier10.1590/S0074-02761991000100006
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898286
dc.descriptionThe proteins of adults worms (male and female) of two isolates (BH and RJ) of Shistosoma mansoni were extracted using Triton X-114 phase separation. The SDS-polyacrilamide gel electrophoresis profiles of the three phases (detergent, aqueous and insoluble proteins) obtained were compared after Coomassie blue and silver staining, surface radioiodination and Western blotting. No major differences were detected between the 2 isolates. Of the 25 or more proteins which partitioned into the detergent phase, only about 8 proteins could be surface radiodinated on live adult worms. A comparison was also made between the profiles of mael and females worms, isolated from bisexually infected mice. Two major female-specific and one male-specific band were detected by silver and/or Coomassie staining. The female bands, 32 KDa and 18 KDa, partitioned into the detergent and aqueous phase, respectively. The male-specific band of 42 KDa remained in the insoluble phase. Antigenic differences between male and females protins were detected by Western vlotting using a sera from infected Nectomys squamipes.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectSchistosoma mansoni
dc.subjectIdentificação
dc.subjectClassificação
dc.subjectProteínas ligadas ao sexo
dc.subjectSex-linked proteins
dc.subjectSchistosoma mansoni
dc.subjectIdentification
dc.subjectClassification
dc.titleIdentification and characterization of sex-linked proteins of Schistosoma mansoni
dc.typeArticle


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