ETV4 plays a role on the primary events during the adenoma-adenocarcinoma progression in colorectal cancer

dc.creatorFonseca, Aline Simoneti
dc.creatorRamão, Anelisa
dc.creatorBürger, Matheus Carvalho
dc.creatorSouza, Jorge Estefano Santana de
dc.creatorZanette, Dalila Lucíola
dc.creatorMolfetta, Greice Andreotti de
dc.creatorAraújo, Luiza Ferreira de
dc.creatorBueno, Rafaela de Barros e Lima
dc.creatorAguiar, Graziela Moura
dc.creatorPlaça, Jessica Rodrigues
dc.creatorAlves, Cleidson de Pádua
dc.creatorSantos, Anemari Ramos Dinarte dos
dc.creatorVidal, Daniel Onofre
dc.creatorSilva, Gyl Eanes Barros
dc.creatorPanepucci, Rodrigo Alexandre
dc.creatorPeria, Fernanda Maris
dc.creatorFeres, Omar
dc.creatorRocha, José Joaquim Ribeiro da
dc.creatorZago, Marco Antonio
dc.creatorSilva Júnior, Wilson Araújo
dc.date2021-04-27T19:35:13Z
dc.date2021-04-27T19:35:13Z
dc.date2021
dc.date.accessioned2023-09-27T00:10:58Z
dc.date.available2023-09-27T00:10:58Z
dc.identifierFONSECA, Aline Simoneti et al. ETV4 plays a role on the primary events during the adenoma-adenocarcinoma progression in colorectal cancer. BMC Cancer, v.21, n. 207, p. 1–14, 2021.
dc.identifier1471-2407
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/46942
dc.identifier10.1186/s12885-021-07857-x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898270
dc.descriptionColorectal cancer (CRC) is one of the most common cancers worldwide; it is the fourth leading cause of death in the world and the third in Brazil. Mutations in the APC, DCC, KRAS and TP53 genes have been associated with the progression of sporadic CRC, occurring at defined pathological stages of the tumor progression and consequently modulating several genes in the corresponding signaling pathways. Therefore, the identification of gene signatures that occur at each stage during the CRC progression is critical and can present an impact on the diagnosis and prognosis of the patient. In this study, our main goal was to determine these signatures, by evaluating the gene expression of paired colorectal adenoma and adenocarcinoma samples to identify novel genetic markers in association to the adenoma-adenocarcinoma stage transition. As methods: ten paired adenoma and adenocarcinoma colorectal samples were subjected to microarray gene expression analysis. In addition, mutations in APC, KRAS and TP53 genes were investigated by DNA sequencing in paired samples of adenoma, adenocarcinoma, normal tissue, and peripheral blood from ten patients. In the results, gene expression analysis revealed a signature of 689 differentially expressed genes (DEG) (fold-change> 2, p< 0.05), between the adenoma and adenocarcinoma paired samples analyzed. Gene pathway analysis using the 689 DEG identified important cancer pathways such as remodeling of the extracellular matrix and epithelialmesenchymal transition. Among these DEG, the ETV4 stood out as one of the most expressed in the adenocarcinoma samples, further confirmed in the adenocarcinoma set of samples from the TCGA database. Subsequent in vitro siRNA assays against ETV4 resulted in the decrease of cell proliferation, colony formation and cell migration in the HT29 and SW480 colorectal cell lines. DNA sequencing analysis revealed KRAS and TP53 gene pathogenic mutations, exclusively in the adenocarcinomas samples. This way, our study identified a set of genes with high potential to be used as biomarkers in CRC, with a special emphasis on the ETV4 gene, which demonstrated involvement in proliferation and migration.
dc.formatapplication/pdf
dc.languagepor
dc.publisherBMC
dc.rightsopen access
dc.subjectCâncer Colorretal
dc.subjectETV4
dc.subjectColorectal Neoplasms
dc.subjectGenetic Markers
dc.subjectNeoplasias Colorrectales
dc.subjectMarcadores Genéticos
dc.subjectAdénocarcinome
dc.subjectAdénomes
dc.subjectTumeurs colorectales
dc.subjectMarqueurs génétiques
dc.subjectAdenocarcinoma
dc.subjectAdenoma
dc.subjectNeoplasias Colorretais
dc.subjectMarcadores Genéticos
dc.titleETV4 plays a role on the primary events during the adenoma-adenocarcinoma progression in colorectal cancer
dc.typeArticle


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