Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism

dc.creatorCaldeira, Roberta Lima
dc.creatorCarvalho, Omar dos Santos
dc.creatorMendonça, Cristiane Lafetá Furtado de
dc.creatorTeixeira, Carlos Graeff
dc.creatorSilva, Márcia C. F.
dc.creatorBen, Renata
dc.creatorMaurer, Rafael
dc.creatorLima, Walter S.
dc.creatorLenzi, Henrique Leonel
dc.date2012-02-28T16:14:04Z
dc.date2012-02-28T16:14:04Z
dc.date2003
dc.date.accessioned2023-09-27T00:10:47Z
dc.date.available2023-09-27T00:10:47Z
dc.identifierCALDEIRA, Roberta L. et al. Molecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction-restriction fragment length polymorphism. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 8, p. 1039-1043, 2003.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/3784
dc.identifier10.1590/S0074-02762003000800011
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898237
dc.descriptionAcknowledgements to Dr Kentaro Yoshimura, Department of Parasitology, Medical School of University of Akita, Japan, for providing us with Angiostrongylus cantonensis; Joziana Barçante, Department of Parasitology of Federal University of Minas Gerais, Brazil, for maintaining the life cycle of A. vasorum; Ester Maria Mota, Department of Pathology, Oswaldo Cruz Institute-Fiocruz, Rio de Janeiro, Brazil, for being in charge of A. costaricensis life cycle.
dc.descriptionAngiostrongylus cantonensis, A. costaricensis, and A. vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection of vertebrate hosts with the third-stage larvae. In order to obtain a diagnostic tool to differentiate larvae and adult worm from the three referred species, polymerase chain reaction-restriction fragment length polymorphism was carried out. The rDNA second internal transcribed spacer (ITS2) and mtDNA cytochrome oxidase I regions were amplified, followed by digestion of fragments with the restriction enzymes RsaI, HapII, AluI, HaeIII, DdeI and ClaI. The enzymes RsaI and ClaI exhibited the most discriminating profiles for the differentiation of the regions COI of mtDNA and ITS2 of rDNA respectively. The methodology using such regions proved to be efficient for the specific differentiation of the three species of Angiostrongylus under study.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectAngiostrongylus
dc.subjectPolymerase chain reaction
dc.subjectRestriction fragment length polymorphism
dc.subjectSecond internal transcribed spacer
dc.subjectCytochrome oxidase I
dc.subjectAngiostrongylus
dc.subjectReação em Cadeia da Polimerase
dc.subjectPolimorfismo de Fragmento de Restrição
dc.subjectCitocromos
dc.titleMolecular differentiation of Angiostrongylus costaricensis, A. cantonensis, and A. vasorum by polymerase chain reaction- restriction fragment length polymorphism
dc.typeArticle


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