Brasil | Article

Applicability of a novel immunoassay based on surface plasmon resonance for the diagnosis of Chagas disease

dc.creatorGuimarães, Gabriel
dc.creatorSouto, Dênio Emanuel Pires
dc.creatorAssis, Girley Francisco Machado
dc.creatorLana, Marta de
dc.creatorLuz, Rita de Cassia Silva
dc.creatorMartins Filho, Olindo Assis
dc.creatorDamos, Flávio Santos
dc.creatorMartins, Helen Rodrigues
dc.date2016-10-05T18:58:29Z
dc.date2016-10-05T18:58:29Z
dc.date2016
dc.date.accessioned2023-09-27T00:10:39Z
dc.date.available2023-09-27T00:10:39Z
dc.identifierGUIMARÃES, Gabriel et al. Applicability of a novel immunoassay based on surface plasmon resonance for the diagnosis of Chagas disease. Clin Chim Acta, vol. 454, p. 39-45, 2016.
dc.identifier0009-8981
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16106
dc.identifier10.1016/j.cca.2015.12.025
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898215
dc.descriptionBACKGROUND: We defined the methodological criteria for the interpretation of the results provided by a novel immunoassay based on surface plasmon resonance (SPR) to detect antibodies anti-Trypanosoma cruzi in human sera (SPRCruzi). Then, we evaluated its applicability as a diagnostic tool for Chagas disease. METHODS: To define the cut-off point and serum dilution factor, 57 samples were analyzed at SPRCruzi and the obtained values of SPR angle displacement (ΔθSPR) were submitted to statistical analysis. Adopting the indicated criteria, its performance was evaluated into a wide panel of samples, being 99 Chagas disease patients, 30 non-infected subjects and 42 with other parasitic/infectious diseases. In parallel, these samples were also analyzed by ELISA. RESULTS: Our data demonstrated that 1:320 dilution and cut-off point at ∆θSPR=17.2 m° provided the best results. Global performance analysis demonstrated satisfactory sensitivity (100%), specificity (97.2%), positive predictive value (98%), negative predictive value (100%) and global accuracy (99.6%). ELISA and SPRCruzi showed almost perfect agreement, mainly between chagasic and non-infected individuals. However, the new immunoassay was better in discriminate Chagas disease from other diseases. CONCLUSION: This work demonstrated the applicability of SPRCruzi as a feasible, real time, label free, sensible and specific methodology for the diagnosis of Chagas disease.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Scientific Pub. Co
dc.rightsrestricted access
dc.subjectDoença de chagas
dc.subjectApplicability
dc.subjectChagas disease
dc.subjectDiagnosis
dc.subjectImmunoassay
dc.subjectSurface plasmon resonance
dc.titleApplicability of a novel immunoassay based on surface plasmon resonance for the diagnosis of Chagas disease
dc.typeArticle


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