IgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosis

dc.creatorJesus, Laura Néspoli Nassar Pansini de
dc.creatorTonini, Aline de Castro Zacche
dc.creatorBarros, Geisa Baptista
dc.creatorReis, Jordana Grazziela Alves Coelho dos
dc.creatorBéla, Samantha Ribeiro
dc.creatorAntonelli, Lis Ribeiro do Valle
dc.creatorMachado, Anderson Silva
dc.creatorCarneiro, Ana Carolina Aguiar Vasconcelos
dc.creatorAndrade, Gláucia Manzan Queiroz
dc.creatorSantos, Daniel Vitor Vasconcelos
dc.creatorJanuário, José Nélio
dc.creatorCarvalho, Andréa Teixeira de
dc.creatorVitor, Ricardo Wagner Almeida
dc.creatorFerro, Eloísa Amália Vieira
dc.creatorMineo, José Roberto
dc.creatorOliveira, Lilian Maria Garcia Bahia
dc.creatorMartins Filho, Olindo Assis
dc.creatorLemos, Elenice Moreira
dc.date2016-10-05T17:44:36Z
dc.date2016-10-05T17:44:36Z
dc.date2016
dc.date.accessioned2023-09-27T00:10:33Z
dc.date.available2023-09-27T00:10:33Z
dc.identifierJESUS, Laura Néspoli Nassar Pansini de et al. IgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosis. J Immunol Methods. , vol. 428, p. 1-8, 2016.
dc.identifier0022-1759
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16098
dc.identifier10.1016/j.jim.2015.11.004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898196
dc.descriptionThis study intended to apply the flow cytometric analysis of IgA and IgG reactivity and intracytoplasmic cytokine analysis to understand and decode the clinical aspects of infants with ocular congenital toxoplasmosis. The Toxoplasma gondii-infected infants (TOXO) were subdivided according to their clinical aspects based on the absence (NRL), presence of active (ARL), active/cicatricial (ACRL) or cicatricial retinochoroidal lesions (CRL) and compared to non-infected controls (NI). The reactivity of anti-T. gondii IgG subclasses resembles the clinical aspects of ocular lesions. IgG and IgG1 discriminate infants with cicatricial lesions (ACRL and CRL) from both ARL and NLR. IgG2 and IgG3 are particularly higher in ACRL and CRL as compared to NLR. No differences were observed when IgG4 reactivity was evaluated. Thus, the results indicated that the reactivity patterns of IgA, IgG and IgG subclasses are able to discriminate ARL, ACRL and CRL from NLR or NI. IgA and IgG subclasses are relevant serological biomarkers with diagnostic and prognostic applicability, respectively. Moreover, IgA and IgG1 were closely related to cytokine production by innate/adaptive immunity cells. IgA reactivity was directly associated to TNF-α-derived from neutrophils, monocytes and CD8(+) T-cells, while IgG1 was inversely correlated with IFN-γ-producing CD4(+) and CD8(+) T-cells but positively correlated with IL-10(+) B-cells. These findings provide insights on the relationship between the cytokine production by innate/adaptive immunity and the antibody pattern of infants with ocular congenital toxoplasmosis. In addition, the present study supports the use of flow cytometric serology as a potential tool for the diagnosis and monitoring of ocular lesions in T. gondii-infected infants in the clinical setting.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Scientific Pub. Co
dc.rightsrestricted access
dc.subjectIgA
dc.subjectIgG1
dc.subjectCytokines
dc.subjectIgA
dc.subjectIgG1
dc.subjectOcular congenital toxoplasmosis
dc.subjectT. gondii
dc.titleIgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosis
dc.typeArticle


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