Development of a rapid and sensitive latex agglutination-based method for detection of group A rotavirus

dc.creatorGóes, Ana Carolina Magalhães Andrade de
dc.creatorMoraes, Marcia Terezinha Baroni de
dc.creatorSilveira, Waldemir de Castro
dc.creatorAraújo, Irene Trigueiros
dc.creatorH'alluin, Jean-Claude de
dc.creatorSouza, Waldemar da Silva
dc.creatorSilva Junior, José Godinho da
dc.creatorLeite, José Paulo Gagliardi
dc.date2018-08-02T17:44:10Z
dc.date2018-08-02T17:44:10Z
dc.date2008
dc.date.accessioned2023-09-27T00:10:32Z
dc.date.available2023-09-27T00:10:32Z
dc.identifierGÓES, Ana Carolina Magalhães Andrade de; et al. Development of a rapid and sensitive latex agglutination-based method for detection of group A rotavirus. Journal of Virological Methods, v.148, n.2, p.211-217, 2008.
dc.identifier0166-0934
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27815
dc.identifier10.1016/j.jviromet.2007.11.013
dc.identifier1879-0984
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898195
dc.descriptionConsidering the background of morbidity and mortality caused by human rotavirus, detection methods that use rotavirus group antigen (VP6) in either enzyme immunoassay (EIA) or latex agglutination test (LAT) has been employed routinely in clinical diagnostic and epidemiological studies. In order to develop a rapid and sensitive rotavirus group A LAT, part of segment 6 corresponding to conserved N-terminal portion of the VP6 (1-245 aa) was cloned in Escherichia coli expression pGEX vector (glutathione S-transferase-GST gene fusion system) that has been modified previously containing a histidine tail at C-terminus. The immunological propriety of the recombinant VP6 having a total molecular weight of 52 kDa was evaluated by Western blot and by the ability of inducing anti-recombinant VP6 polyclonal antibodies in rabbit. The polyclonal serum produced was conjugated to a latex support to detect rotavirus in stool specimens. The percentage values for sensitivity and specificity of the rotavirus group A LAT were 98.5% and 100%, respectively.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectGastroenterite
dc.subjectRotavírus do grupo A
dc.subjectProteína Recombinante
dc.subjectGastroenteritis
dc.subjectGroup A rotavirus
dc.subjectRecombinant protein
dc.subjectVP6
dc.subjectLatex
dc.titleDevelopment of a rapid and sensitive latex agglutination-based method for detection of group A rotavirus
dc.typeArticle


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