Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls

dc.creatorBobosha, Kidist
dc.creatorvan der Ploeg-van Schip, Jolien J.
dc.creatorEsquenazi, Danuza A.
dc.creatorGuimarães, Marjorie M.
dc.creatorMartins, Marcia V.
dc.creatorBekele, Yonas
dc.creatorFantahun, Yonas
dc.creatorAseffa, Abraham
dc.creatorFranken, Kees L. M. C.
dc.creatorGismondi, Ronaldo C.
dc.creatorPessolani, Maria C. V.
dc.creatorOttenhoff, Tom H. M.
dc.creatorPereira, Geraldo M. B.
dc.creatorGeluk, Annemieke
dc.date2017-04-11T17:08:07Z
dc.date2017-04-11T17:08:07Z
dc.date2012
dc.date.accessioned2023-09-27T00:10:31Z
dc.date.available2023-09-27T00:10:31Z
dc.identifierBOBOSHA, Kidist; et al. Peptides Derived fromMycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls. Journal of Tropical Medicine, Volume 2012, Article ID132049, 11p, 2012.
dc.identifier1687-9686
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18383
dc.identifier10.1155/2012/132049
dc.identifier1687-9694
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898191
dc.descriptionThe stable incidence of new leprosy cases suggests that transmission of infection continues despite worldwide implementation of MDT. Thus, specific tools are needed to diagnose early stage Mycobacterium leprae infection, the likely sources of transmission. M. leprae antigens that induce T-cell responses in M. leprae exposed and/or infected individuals thus are major targets for new diagnostic tools. Previously, we showed that ML1601c was immunogenic in patients and healthy household contacts (HHC). However, some endemic controls (EC) also recognized this protein. To improve the diagnostic potential, IFN-γ responses to ML1601c peptides were assessed using PBMC from Brazilian leprosy patients and EC. Five ML1601c peptides only induced IFN-γ in patients and HHC. Moreover, 24-hour whole-blood assay (WBA), two ML1601c peptides could assess the level of M. leprae exposure in Ethiopian EC. Beside IFN-γ, also IP-10, IL-6, IL-1β, TNF-α, and MCP-1 were increased in EC from areas with high leprosy prevalence in response to these ML1601c peptides. Thus, ML1601c peptides may be useful for differentiating M. leprae exposed or infected individuals and can also be used to indicate the magnitude of M. leprae transmission even in the context of various HLA alleles as present in these different genetic backgrounds.
dc.formatapplication/pdf
dc.languageeng
dc.publisherHindawi Publishing Corporation
dc.rightsopen access
dc.subjectMycobacterium leprae
dc.subjectPeptídeos
dc.subjectHanseníase
dc.subjectControles endêmicos
dc.subjectMycobacterium leprae
dc.subjectPeptides
dc.subjectLeprosy
dc.subjectEndemic Controls
dc.titlePeptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls
dc.typeArticle


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