Effects of a putrescine analog on Giardia lamblia

dc.creatorMaia, Claudia
dc.creatorRangel, Adriana Lanfredi
dc.creatorAnjos, Karla Graziela Santana dos
dc.creatorOliveira, Marcus Fernandes de
dc.creatorSouza, Wanderley de
dc.creatorSantos, Marcos André Vannier dos
dc.date2016-07-21T12:29:14Z
dc.date2016-07-21T12:29:14Z
dc.date2008
dc.date.accessioned2023-09-27T00:10:30Z
dc.date.available2023-09-27T00:10:30Z
dc.identifierMAIA, C. et al. Effects of a putrescine analog on Giardia lamblia. Parasitology Research, v. 103, p. 363–370, 2008.
dc.identifier0932-0113
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15020
dc.identifier10.1007/s00436-008-0981-9
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898188
dc.descriptionThe protozoan Giardia lamblia is the most frequent intestinal parasite of first-world countries and a major cause of waterborne disorder often referred to as traveler's diarrhea. We have previously noticed that the putrescine analog 1,4-diamino-2-butanone (DAB) remarkably inhibits the growth of anaerobic trichomonad and Trypanosoma cruzi parasites. Here, we examined the role of polyamines in Giardia cells using this putrescine analog. DAB impaired parasite proliferation dose-dependently. The analog induced increased flagella numbers and sometimes four ventral disks as well as asymmetrical division, indicating truncated or deregulated cytokinesis. Electron microscopy analysis revealed that DAB also triggered the encystment process. Oxidative stress was evaluated by measuring lipid peroxidation by thiobarbituric acid reactive substances (TBARS) detection. Trophozoites incubated either with 1 mM of DAB or putrescine for 18 h displayed increased lipoperoxide levels. Addition of 200 microM aminoguanidine, a polyamine/diamine oxidase inhibitor, partially reverted the DAB, but not the putrescine effects, indicating that the DAB effects are due, at least in part, to DAB oxidation end products. These data indicate that polyamines play a role in Giardia cell division, differentiation, and antioxidant defenses.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag
dc.rightsopen access
dc.subjectAnimals
dc.subjectGiardia lamblia
dc.subjectOxidative Stress
dc.subjectPolyamines
dc.subjectPutrescine
dc.subjectTrophozoites
dc.titleEffects of a putrescine analog on Giardia lamblia
dc.typeArticle


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