Population hetereogeneity among clones of New World Leishmania species

dc.creatorPacheco, R. S.
dc.creatorCrimaldi Jr, G.
dc.creatorMomen, H.
dc.creatorMorel, Carlos Medicis
dc.date2014-12-10T10:29:33Z
dc.date2018-04-12T16:59:08Z
dc.date2014-12-10T10:29:33Z
dc.date2018-04-12T16:59:08Z
dc.date1990
dc.date.accessioned2023-09-27T00:09:00Z
dc.date.available2023-09-27T00:09:00Z
dc.identifierPACHECO, R. S. et al. Population heterogeneity amomg clones of New World Leishmania species. Parasitology, v.100, p.393-398, 1990
dc.identifier00311820
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25825
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8897931
dc.descriptionCell cloning techniques and schizodeme analysis were used to detect mixtures of subpopulations in Leishmania parasites, isolated from humans and a reservoir host. Clones were obtained by plating promastigotes, at limited dilution, on solid medium. The resultant colonies were analysed by the restriction profile of kinetoplast DNA minicircles (schizodeme analysis). The efficiency of plating was around 40-60%, and subpopulations of different schizodemes, in stocks of Leishmania isolated from 2 cases of human cutaneous leishmaniasis were detected. The presence of different schizodemes in one isolate from a sylvatic animal suggested the possibility of a mixed natural infection.
dc.formatapplication/pdf
dc.languagepor
dc.publisherCambridge University Press
dc.rightsrestricted access
dc.subjectLeishmania
dc.subjectDNA de Cinetoplasto
dc.subjectClonagem de células
dc.subjectcell clonning
dc.subjectkDNA
dc.subjectschizodemes
dc.subjectLeishmania
dc.titlePopulation hetereogeneity among clones of New World Leishmania species
dc.typeArticle


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