Identification of novel proteins from the venom of a cryptic snake Drysdalia coronoides by a combined transcriptomics and proteomics approach

dc.creatorChatrath, Shifali Tiwari
dc.creatorChapeaurouge, Alex
dc.creatorLin, Qingsong
dc.creatorLim, Teck Kwang
dc.creatorDunstan, Nathan
dc.creatorMirtschin, Peter
dc.creatorKumar, Prakash P.
dc.creatorKini, R. Manjunatha
dc.date2018-03-27T17:12:51Z
dc.date2018-03-27T17:12:51Z
dc.date2011
dc.date.accessioned2023-09-27T00:08:45Z
dc.date.available2023-09-27T00:08:45Z
dc.identifierCHATRATH, Shifali Tiwari; et al. Identification of Novel Proteins from the Venom of a Cryptic Snake Drysdalia coronoides by a Combined Transcriptomics and Proteomics Approach. Journal of Proteome Research, v.10, p.739–750, June 2010.
dc.identifier1535-3893
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25530
dc.identifier10.1021/pr1008916
dc.identifier1535-3907
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8897886
dc.descriptionWe have investigated the transcriptome and proteome of the venom of a cryptic Australian elapid snake Drysdalia coronoides. To probe into the transcriptome, we constructed a partial cDNA library from the venom gland of D. coronoides. The proteome of the venom of D. coronoides was explored by tryptic digestion of the crude venom followed by HPLC separation of the resulting peptides and MALDI-TOF/TOF mass spectrometric analysis. Importantly, the tandem MS data of the tryptic peptides of the venom not only confirmed the predicted protein sequences deduced from the transcriptome, but also added to our knowledge about the venom composition through identification of two more toxin families. Using both the approaches, we were able to identify proteins belonging to eight different snake venom protein superfamilies, namely, three-finger toxins, serine protease inhibitors, cysteine rich secretory proteins, phospholipases A(2), venom nerve growth factors, snake venom metalloproteases, vespryns, and a new family phospholipase B. We also identified three novel proteins belonging to the three-finger toxin superfamily.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Chemical Society
dc.rightsrestricted access
dc.subjectTranscriptoma
dc.subjectProteoma
dc.subjectFosfolipases
dc.subjectEspectrometria de Massas em Tandem
dc.subjecttranscriptome
dc.subjectthree-finger toxins
dc.subjectphospholipases
dc.subjectproteome
dc.subjecttandem mass spectrometry
dc.titleIdentification of novel proteins from the venom of a cryptic snake Drysdalia coronoides by a combined transcriptomics and proteomics approach
dc.typeArticle


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