Local Regulation of Adrenal Steroidogenesis: Subtle in vitro Effects of IL-1β on the Human Cell Line NCI-H295R Steroid Production along with Changes in MicroRNA Profile and Orphan Nuclear Receptors NR4As

dc.creatorSantucci, Natalia
dc.creatorStampone, Rocío
dc.creatorSilva, Eduardo Brandão Ferreira da
dc.creatorVillar, Silvina
dc.creatorSpinelli, Silvana
dc.creatorLinhares-Lacerda, Leandra
dc.creatorRibeiro-Alves, Marcelo
dc.creatorBay, María Luisa
dc.creatorBottasso, Oscar
dc.date2021-03-23T12:17:53Z
dc.date2021-03-23T12:17:53Z
dc.date2020
dc.date.accessioned2023-09-27T00:07:21Z
dc.date.available2023-09-27T00:07:21Z
dc.identifierSANTUCCI, Natalia et al. Local Regulation of Adrenal Steroidogenesis: Subtle in vitro Effects of IL-1β on the Human Cell Line NCI-H295R Steroid Production along with Changes in MicroRNA Profile and Orphan Nuclear Receptors NR4As. Neuroimmunomodulation, v. 27, n. 3, p. 131-141, 2020
dc.identifier1423-0216
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/46399
dc.identifier10.1159/000512325
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8897649
dc.descriptionIntroduction: IL-1β, a cytokine from the innate immune response, is well known for its proinflammatory effects and stimulating activity on the hypothalamus-pituitary-adrenal axis, leading to the pituitary synthesis of adrenocorticotropic hormone followed by cortisol (and dehydroepiandrosterone - DHEA) release by the adrenal gland. While IL-1β modulates the adrenal steroidogenesis at the central level, it is unclear whether it also exerts an effect on the adrenal gland. Method: We studied the effect of IL-1β on adrenal steroid production and steroidogenic enzyme RNA expression in the human cell line NCI-H295R. We also explored eventual changes in the microRNA (miRNA) profile from IL-1β-treated NCI-H295R cells. Results: Transcripts encoding IL-1β receptors 1 and 2 were noticeable in the cell line, with cortisol and DHEA production showing a subtle increase after cytokine treatment. Transcripts from key enzymes in the steroidogenic pathway were analyzed, with no noticeable changes on them. The miRNA profile was modified by IL-1β treatment to an extent which bears some relationship with the regulatory mechanisms underlying adrenal steroid production. Since orphan nuclear receptors NR4As have emerged as potential key factors for coordinating inflammatory and metabolic responses, cell expression studies were also carried out to show an NR4As transcript augmentation following IL-1β treatment. Discussion/conclusions: The subtle increase in adrenal steroid production in response to IL-1β stimulation without any modification in the transcription of the steroidogenic enzymes analyzed suggests an additional inflammatory/anti-inflammatory loop, wherein NR4As receptors may participate. Besides its physiological role, this process might be implied in pathological states accompanied by an unbalanced immune-endocrine relationship.
dc.formatapplication/pdf
dc.languageeng
dc.publisherKarger
dc.rightsrestricted access
dc.subjectAdrenal steroidogenesis
dc.subjectCortisol
dc.subjectDehydroepiandrosterone
dc.subjectInterleukin 1β
dc.subjectNR4As
dc.subjectmiRNA
dc.titleLocal Regulation of Adrenal Steroidogenesis: Subtle in vitro Effects of IL-1β on the Human Cell Line NCI-H295R Steroid Production along with Changes in MicroRNA Profile and Orphan Nuclear Receptors NR4As
dc.typeArticle


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