Potential biomarkers for the clinical prognosis of severe dengue

dc.creatorSilva, Mayara Marques Carneiro da
dc.creatorGil, Laura Helena Vega Gonzales
dc.creatorMarques, Ernesto Torres de Azevedo
dc.creatorCalzavara-Silva, Carlos Eduardo
dc.date2017-12-12T12:41:16Z
dc.date2017-12-12T12:41:16Z
dc.date2013
dc.date.accessioned2023-09-27T00:07:08Z
dc.date.available2023-09-27T00:07:08Z
dc.identifierSILVA, M. M. C. da et al. Potential biomarkers for the clinical prognosis of severe dengue. Memorias Do Instituto Oswaldo Cruz, v. 108, n. 6, p. 755–762, set. 2013.
dc.identifier1678-8060
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23617
dc.identifier10.1590/0074-0276108062013012
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8897622
dc.descriptionCurrently, several assays can confirm acute dengue infection at the point-of-care. However, none of these assays can predict the severity of the disease symptoms. A prognosis test that predicts the likelihood of a dengue patient to develop a severe form of the disease could permit more efficient patient triage and treatment. We hypothesise that mRNA expression of apoptosis and innate immune response-related genes will be differentially regulated during the early stages of dengue and might predict the clinical outcome. Aiming to identify biomarkers for dengue prognosis, we extracted mRNA from the peripheral blood mononuclear cells of mild and severe dengue patients during the febrile stage of the disease to measure the expression levels of selected genes by quantitative polymerase chain reaction. The selected candidate biomarkers were previously identified by our group as differentially expressed in microarray studies. We verified that the mRNA coding for CFD, MAGED1, PSMB9, PRDX4 and FCGR3B were differentially expressed between patients who developed clinical symptoms associated with the mild type of dengue and patients who showed clinical symptoms associated with severe dengue. We suggest that this gene expression panel could putatively serve as biomarkers for the clinical prognosis of dengue haemorrhagic fever.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectdengue
dc.subjectbiomarcadores
dc.subjectapoptose
dc.subjectimunidade inata
dc.subjectPCR quantitativa em tempo real
dc.subjectDengue
dc.subjectbiomarkers
dc.subjectapoptosis
dc.subjectinnate immunity
dc.subjectquantitative real-time PCR
dc.subjectAntígenos de Neoplasias
dc.subjectgenética
dc.subjectCisteína Endopeptidases
dc.subjectgenética
dc.subjectGlicoproteínas de Membrana
dc.subjectgenética
dc.subjectProteínas de Neoplasias
dc.subjectgenética
dc.subjectPeroxirredoxinas
dc.subjectgenética
dc.subjectReceptores de IgG
dc.subjectgenética
dc.subjectReceptores de Interleucina-1
dc.subjectgenética
dc.subjectDengue Grave
dc.subjectdiagnóstico
dc.subjectÍndice de Gravidade de Doença
dc.subjectBiomarcadores
dc.subjectProteínas Reguladoras de Apoptose
dc.subjectgenética
dc.subjectProteínas Ligadas por GPI
dc.subjectgenética
dc.subjectExpressão Gênica
dc.subjectHumanos
dc.subjectImunidade Inata
dc.subjectgenética
dc.subjectLeucócitos Mononucleares
dc.subjectimunologia
dc.subjectLeucócitos Mononucleares
dc.subjectpatologia
dc.subjectAnálise em Microsséries
dc.subjectPrognóstico
dc.subjectRNA Mensageiro
dc.subjectisolamento & purificação
dc.subjectReação em Cadeia da Polimerase em Tempo Real
dc.subjectSorotipagem
dc.titlePotential biomarkers for the clinical prognosis of severe dengue
dc.typeArticle


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