Phylogenetic analysis reveals a high prevalence of Sporothrix brasiliensis in feline sporotrichosis outbreaks

dc.creatorRodrigues, Anderson Messias
dc.creatorTeixeira, Marcus de Melo
dc.creatorHoog, G. Sybren de
dc.creatorSchubach, Tânia Maria Pacheco
dc.creatorPereira, Sandro Antonio
dc.creatorFernandes, Geisa Ferreira
dc.creatorBezerra, Leila Maria Lopes
dc.creatorFelipe, Maria Sueli
dc.creatorCamargo, Zoilo Pires de
dc.date2019-05-24T14:10:29Z
dc.date2019-05-24T14:10:29Z
dc.date2013
dc.date.accessioned2023-09-27T00:04:30Z
dc.date.available2023-09-27T00:04:30Z
dc.identifierRODRIGUES, Anderson Messias et al. Phylogenetic analysis reveals a high prevalence of Sporothrix brasiliensis in feline Sporotrichosis Outbreaks. PLoS Neglected Tropical Diseases, v. 20; n. 7, p. 1-14, June 2013.
dc.identifier1935-2727
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33207
dc.identifier10.1371/journal.pntd.0002281
dc.identifier1935-2735
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8897168
dc.descriptionSporothrix schenckii, previously assumed to be the sole agent of human and animal sporotrichosis, is in fact a species complex. Recently recognized taxa include S. brasiliensis, S. globosa, S. mexicana, and S. luriei, in addition to S. schenckii sensu stricto. Over the last decades, large epidemics of sporotrichosis occurred in Brazil due to zoonotic transmission, and cats were pointed out as key susceptible hosts. In order to understand the eco-epidemiology of feline sporotrichosis and its role in human sporotrichosis a survey was conducted among symptomatic cats. Prevalence and phylogenetic relationships among feline Sporothrix species were investigated by reconstructing their phylogenetic origin using the calmodulin (CAL) and the translation elongation factor-1 alpha (EF1α) loci in strains originated from Rio de Janeiro (RJ, n = 15), Rio Grande do Sul (RS, n = 10), Paraná (PR, n = 4), São Paulo (SP, n =3) and Minas Gerais (MG, n = 1). Our results showed that S. brasiliensis is highly prevalent among cats (96.9%) with sporotrichosis, while S. schenckii was identified only once. The genotype of Sporothrix from cats was found identical to S. brasiliensis from human sources confirming that the disease is transmitted by cats. Sporothrix brasiliensis presented low genetic diversity compared to its sister taxon S. schenckii. No evidence of recombination in S. brasiliensis was found by split decomposition or PHI-test analysis, suggesting that S. brasiliensis is a clonal species. Strains recovered in states SP, MG and PR share the genotype of the RJ outbreak, different from the RS clone. The occurrence of separate genotypes among strains indicated that the Brazilian S. brasiliensis epidemic has at least two distinct sources. We suggest that cats represent a major host and the main source of cat and human S. brasiliensis infections in Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectSporothrix brasiliensis
dc.subjectSporothrix schenckii
dc.subjectSporotrichosis
dc.subjectFeline sporotrichosis
dc.subjectSporotrichosis outbreaks
dc.titlePhylogenetic analysis reveals a high prevalence of Sporothrix brasiliensis in feline sporotrichosis outbreaks
dc.typeArticle


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