Low ethanol consumption induces enhancement of insulin sensitivity in liver of normal rats

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFuruya, Daniela Tomie
dc.creatorBinsack, Ralf
dc.creatorOnishi, Mary Emy
dc.creatorSeraphim, Patricia Monteiro
dc.creatorMachado, Ubiratan Fabres
dc.date2014-05-27T11:21:35Z
dc.date2016-10-25T18:21:01Z
dc.date2014-05-27T11:21:35Z
dc.date2016-10-25T18:21:01Z
dc.date2005-08-26
dc.date.accessioned2017-04-06T01:14:18Z
dc.date.available2017-04-06T01:14:18Z
dc.identifierLife Sciences, v. 77, n. 15, p. 1813-1824, 2005.
dc.identifier0024-3205
dc.identifierhttp://hdl.handle.net/11449/68369
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68369
dc.identifier10.1016/j.lfs.2004.12.046
dc.identifier2-s2.0-23044479330
dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2004.12.046
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889713
dc.descriptionModerate amounts of alcohol intake have been reported to have a protective effect on the cardiovascular system and this may involve enhanced insulin sensitivity. We established an animal model of increased insulin sensitivity by low ethanol consumption and here we investigated metabolic parameters and molecular mechanisms potentially involved in this phenomenon. For that, Wistar rats have received drinking water either without (control) or with 3% ethanol for four weeks. The effect of ethanol intake on insulin sensitivity was analyzed by insulin resistance index (HOMA-IR), intravenous insulin tolerance test (IVITT) and lipid profile. The role of liver was investigated by the analysis of insulin signaling pathway, GLUT2 gene expression and tissue glycogen content. Rats consuming 3% ethanol showed lower values of HOMA-IR and plasma free fatty acids (FFA) levels and higher hepatic glycogen content and glucose disappearance constant during the IVITT. Neither the phosphorylation of insulin receptor (IR) and insulin receptor substrate-1 (IRS-1), nor its association with phosphatidylinositol-3-kinase (PI3-kinase), was affected by ethanol. However, ethanol consumption enhanced liver IRS-2 and protein kinase B (Akt) phosphorylation (3 times, P < 0.05), which can be involved in the 2-fold increased (P < 0.05) hepatic glycogen content. The GLUT2 protein content was unchanged. Our findings point out that liver plays a role in enhanced insulin sensitivity induced by low ethanol consumption. © 2005 Elsevier Inc. All rights reserved.
dc.languageeng
dc.relationLife Sciences
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAkt/PKB
dc.subjectHepatic glycogen
dc.subjectInsulin receptor substrate-2
dc.subjectInsulin sensitivity
dc.subjectLow-ethanol intake
dc.subjectalcohol
dc.subjectdrinking water
dc.subjectfatty acid
dc.subjectglucose
dc.subjectglucose transporter 2
dc.subjectglycogen
dc.subjectinsulin
dc.subjectinsulin receptor
dc.subjectinsulin receptor substrate 1
dc.subjectinsulin receptor substrate 2
dc.subjectlipid
dc.subjectphosphatidylinositol 3 kinase
dc.subjectprotein kinase B
dc.subjectalcohol consumption
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectcontrolled study
dc.subjectfatty acid blood level
dc.subjectglucose homeostasis
dc.subjectglucose utilization
dc.subjectglycogen analysis
dc.subjectglycogen liver level
dc.subjectinsulin resistance
dc.subjectinsulin sensitivity
dc.subjectintravenous glucose tolerance test
dc.subjectlipid analysis
dc.subjectliver function
dc.subjectmale
dc.subjectmetabolic parameters
dc.subjectnonhuman
dc.subjectprotein content
dc.subjectprotein folding
dc.subjectprotein phosphorylation
dc.subjectrat
dc.subject1-Phosphatidylinositol 3-Kinase
dc.subjectAlcohol Drinking
dc.subjectAnimals
dc.subjectBlotting, Northern
dc.subjectBlotting, Western
dc.subjectBody Weight
dc.subjectCholesterol
dc.subjectEating
dc.subjectEthanol
dc.subjectFatty Acids, Nonesterified
dc.subjectGene Expression
dc.subjectGlucose Transporter Type 2
dc.subjectGlycogen
dc.subjectInsulin
dc.subjectInsulin Resistance
dc.subjectIntracellular Signaling Peptides and Proteins
dc.subjectLipoproteins
dc.subjectLiver
dc.subjectMale
dc.subjectMonosaccharide Transport Proteins
dc.subjectPhosphoproteins
dc.subjectPhosphorylation
dc.subjectProtein-Serine-Threonine Kinases
dc.subjectProto-Oncogene Proteins
dc.subjectProto-Oncogene Proteins c-akt
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReceptor, Insulin
dc.subjectTriglycerides
dc.subjectAnimalia
dc.subjectRattus norvegicus
dc.titleLow ethanol consumption induces enhancement of insulin sensitivity in liver of normal rats
dc.typeOtro


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