Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorda Rosa Guterres, Zaira
dc.creatorMantovani, Mário Sérgio
dc.creatorda Eira, Augusto Ferreira
dc.creatorRibeiro, Lúcia Regina
dc.creatorJordão, Berenice Quinzani
dc.date2014-05-27T11:21:22Z
dc.date2016-10-25T18:20:54Z
dc.date2014-05-27T11:21:22Z
dc.date2016-10-25T18:20:54Z
dc.date2005-07-01
dc.date.accessioned2017-04-06T01:13:49Z
dc.date.available2017-04-06T01:13:49Z
dc.identifierGenetics and Molecular Biology, v. 28, n. 3, p. 458-463, 2005.
dc.identifier1415-4757
dc.identifier1678-4685
dc.identifierhttp://hdl.handle.net/11449/68309
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68309
dc.identifier10.1590/S1415-47572005000300022
dc.identifierS1415-47572005000300022
dc.identifierWOS:000235653200021
dc.identifier2-s2.0-30544454303.pdf
dc.identifier2-s2.0-30544454303
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572005000300022
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889663
dc.descriptionAgaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 → 6)β-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated phases); b) a mixture taken from AB 96/07, AB 96/09 and AB 97/ 11 strains; and c) commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells) were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS). Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS. Copyright by the Brazilian Society of Genetics.
dc.languageeng
dc.relationGenetics and Molecular Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAgaricus blazei
dc.subjectComet assay
dc.subjectMicronuclei
dc.subjectMushroom extracts
dc.subjectV79 cells
dc.subjectAgaricus blazei extract
dc.subjectalcohol
dc.subjectchloroform
dc.subjectmesylic acid methyl ester
dc.subjectanimal cell
dc.subjectcell protection
dc.subjectcell strain V 79
dc.subjectcomet assay
dc.subjectcontrolled study
dc.subjectdrug activity
dc.subjectdrug effect
dc.subjectdrug exposure
dc.subjectdrug isolation
dc.subjectfungal strain
dc.subjectgenotoxicity
dc.subjectin vitro study
dc.subjectmicronucleus
dc.subjectmushroom
dc.subjectmutagenicity
dc.subjectnonhuman
dc.subjectAnimalia
dc.subjectBasidiomycota
dc.subjectCricetulus griseus
dc.titleGenotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells
dc.typeOtro


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