Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries

dc.creatorCools, Piet
dc.creatorVlaminck, Johnny
dc.creatorAlbonico, Marco
dc.creatorAme, Shaali
dc.creatorAyana, Mio
dc.creatorAntonio, Barrios Perez José
dc.creatorCringoli, Giuseppe
dc.creatorDana, Daniel
dc.creatorKeiser, Jennifer
dc.creatorMaurelli, Maria P
dc.creatorMaya, Catalina
dc.creatorMatoso, Leonardo F
dc.creatorMontresor, Antonio
dc.creatorMekonnen, Zeleke
dc.creatorMirams, Greg
dc.creatorOliveira, Rodrigo Corrêa de
dc.creatorPinto, Simone A
dc.creatorRinaldi, Laura
dc.creatorSayasone, Somphou
dc.creatorThomas, Eurion
dc.creatorVerweij, Jaco J
dc.creatorVercruysse, Jozef
dc.creatorLevecke, Bruno
dc.date2023-03-31T13:17:21Z
dc.date2023-03-31T13:17:21Z
dc.date2019
dc.date.accessioned2023-09-27T00:00:06Z
dc.date.available2023-09-27T00:00:06Z
dc.identifierCOOLS, Piet et al. Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. PLoS Negl Trop Dis., v. 13, n. 8, e0007446, 2019. doi: 10.1371/journal.pntd.0007446.
dc.identifier1935-2735
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/57635
dc.identifier10.1371/journal.pntd.0007446
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8896411
dc.descriptionBackground: Because the success of deworming programs targeting soil-transmitted helminths (STHs) is evaluated through the periodically assessment of prevalence and infection intensities, the use of the correct diagnostic method is of utmost importance. The STH community has recently published for each phase of a deworming program the minimal criteria that a potential diagnostic method needs to meet, the so-called target product profiles (TPPs). Methodology: We compared the diagnostic performance of a single Kato-Katz (reference method) with that of other microscopy-based methods (duplicate Kato-Katz, Mini-FLOTAC and FECPAK(G2)) and one DNA-based method (qPCR) for the detection and quantification of STH infections in three drug efficacy trials in Ethiopia, Lao PDR, and Tanzania. Furthermore, we evaluated a selection of minimal diagnostic criteria of the TPPs. Principal findings: All diagnostic methods showed a clinical sensitivity of >= 90% for all STH infections of moderate-to-heavy intensities. For infections of very low intensity, only qPCR resulted in a sensitivity that was superior to a single Kato-Katz for all STHs. Compared to the reference method, both Mini-FLOTAC and FECPAK(G2) resulted in significantly lower fecal egg counts for some STHs, leading to a substantial underestimation of the infection intensity. For qPCR, there was a positive significant correlation between the egg counts of a single Kato-Katz and the DNA concentration. Conclusions/Significance: Our results indicate that the diagnostic performance of a single Kato-Katz is underestimated by the community and that diagnostic specific thresholds to classify intensity of infection are warranted for Mini-FLOTAC, FECPAK(G2) and qPCR. When we strictly apply the TPPs, Kato-Katz is the only microscopy-based method that meets the minimal diagnostic criteria for application in the planning, monitoring and evaluation phase of an STH program. qPCR is the only method that could be considered in the phase that aims to seek confirmation for cessation of program.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.titleDiagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAK(G2) and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries
dc.typeArticle


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