Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C

dc.creatorNakatani, Sueli M.
dc.creatorSantos, Carlos A.
dc.creatorRiediger, Irina Nastassja
dc.creatorKrieger, Marco Aurélio
dc.creatorDuarte, Cesar A. B.
dc.creatorDebur, Maria do Carmo
dc.creatorCarrilho, Flair J.
dc.creatorOno, Suzane K.
dc.date2020-02-11T19:48:16Z
dc.date2020-02-11T19:48:16Z
dc.date2011
dc.date.accessioned2023-09-26T23:59:49Z
dc.date.available2023-09-26T23:59:49Z
dc.identifierNAKATANI, Sueli M. et al. Comparative performance evaluation of hepatitis C virus genotyping based on the 5’ untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C. Virology Journal, v. 8, n. 459, p. 1-5, 2011.
dc.identifier1743-422X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/39884
dc.identifier10.1186/1743-422X-8-459
dc.identifier1743-422X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8896361
dc.descriptionGenotyping of hepatitis C virus (HCV) has become an essential tool for prognosis and prediction of treatment duration. The aim of this study was to compare two HCV genotyping methods: reverse hybridization line probe assay (LiPA v.1) and partial sequencing of the NS5B region. As methods: Plasma of 171 patients with chronic hepatitis C were screened using both a commercial method (LiPA HCV Versant, Siemens, Tarrytown, NY, USA) and different primers targeting the NS5B region for PCR amplification and sequencing analysis. And as results: comparison of the HCV genotyping methods showed no difference in the classification at the genotype level. However, a total of 82/171 samples (47.9%) including misclassification, non-subtypable, discrepant and inconclusive results were not classified by LiPA at the subtype level but could be discriminated by NS5B sequencing. Of these samples, 34 samples of genotype 1a and 6 samples of genotype 1b were classified at the subtype level using sequencing of NS5B. It has as conclusions that: Sequence analysis of NS5B for genotyping HCV provides precise genotype and subtype identification and an accurate epidemiological representation of circulating viral strains.
dc.formatapplication/pdf
dc.languagepor
dc.publisherBMC
dc.rightsopen access
dc.subjectHepacivirus
dc.subjectHepatitis C virus
dc.subjectGenotyping Techniques
dc.subject5' Untranslated Regions
dc.subjectTécnicas de Genotipaje
dc.subjectRegiones no Traducidas 5'
dc.subjectTécnicas de Genotipagem
dc.subjectRegiões 5' não Traduzidas
dc.titleComparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
dc.typeArticle


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