Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases

dc.creatorPylro, Victor Satler
dc.creatorMorais, Daniel Kumazawa
dc.creatorKalks, Karlos Henrique Martins
dc.creatorRoesch, Luiz Fernando Wurdig
dc.creatorHirsch, Penny R.
dc.creatorTótola, Marcos Rogério
dc.creatorYotoko, Karla
dc.date2016-10-18T13:34:58Z
dc.date2016-10-18T13:34:58Z
dc.date2016
dc.date.accessioned2023-09-26T23:58:33Z
dc.date.available2023-09-26T23:58:33Z
dc.identifierPYLRO, Victor Satler et al. Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases. Journal of Microbiological Methods, v. 126, p. 18-23, 2016.
dc.identifier0167-7012
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16269
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8896145
dc.descriptionControversy surrounding bacterial phylogenies has become one of the most important challenges for microbial ecology. Comparative analyses with nucleotide databases and phylogenetic reconstruction of the amplified 16S rRNA genes from DGGE (Denaturing Gradient Gel Electrophoresis) excised bands have been used by several researchers for the identification of organisms in complex samples. Here, we individually analyzed DGGE-excised 16S rRNA gene bands from 10 certified bacterial strains of different species, and demonstrated that this kind of approach can deliver erroneous outcomes to researchers, besides causing/emphasizing errors in public databases.
dc.description2021-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subject16S rRNA gene
dc.subject16S rRNA gene
dc.subjectPCR-DGGE bacteria phylogeny
dc.subjectPublic databases
dc.titleMisguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases
dc.typeArticle


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