Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis

dc.creatorSchallig, Henk D. F. H.
dc.creatorCavalheiro, Marilene Canto
dc.creatorSilva, Eduardo S. da
dc.date2020-10-27T14:26:17Z
dc.date2020-10-27T14:26:17Z
dc.date2002
dc.date.accessioned2023-09-26T23:57:38Z
dc.date.available2023-09-26T23:57:38Z
dc.identifierSCHALLIG, Henk D. F. H.; CAVALHEIRO, Marilene Canto; SILVA, Eduardo S. da. Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 7, p. 1015-1018, Oct. 2002.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44226
dc.identifier10.1590/S0074-02762002000700015
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8896008
dc.descriptionThe direct agglutination test (DAT) based on a freeze-dried antigen and the rK39 dipstick test were evaluated for the sero-diagnosis of visceral leishmaniasis (VL). The sensitivity and specificity of both tests were determined using sera from confirmed VL patients (n = 21), healthy controls (n = 19) and from patients with other confirmed infectious diseases (n = 42). The DAT had a sensitivity and a specificity of 100%. The rK39 had a sensitivity of 85.7% and a specificity of 82%. Both tests were also used to screen blood samples of confirmed VL patients (n = 15) and serum samples of VL suspects (n = 61). The DAT found all blood samples of confirmed VL patients positive and tested 98.4% of the serum samples of the VL suspects positive. In contrast, rK39 detected in 9/15 blood samples (60%) antibodies against Leishmania chagasi and found 85.3% of the serum samples of the suspected patients positive. Although the rK39 dipstick is more rapid and user friendlier than the DAT, the latter has a superior sensitivity and specificity. Furthermore, the reagents used for DAT do not require cold storage, whereas the buffer of the rK39 must be stored at 4oC. Therefore, the DAT is the most suitable test for the sero-diagnosis of VL under field conditions.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectLeishmaniose Visceral
dc.subjectDiagnóstico
dc.subjectLeishmania chagasi
dc.subjectHumanos
dc.subjectTesT de aglutinação direta
dc.subjectTeste de vareta rK39
dc.subjectBrasil
dc.subjectVisceral leishmaniasis
dc.subjectDirect agglutination tesT
dc.subjectrK39 dipstick test
dc.subjectLeishmania chagasi
dc.subjectDiagnosis
dc.subjectHuman
dc.subjectBrazil
dc.titleEvaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
dc.typeArticle


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