Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera

dc.creatorNogueira, Caio Cesar Richter
dc.creatorPaixão, Izabel Christina Nunes de Palmer
dc.creatorSantos, Claudio Cesar Cirne
dc.creatorStephens, Paulo Roberto Soares
dc.creatorVillaça, Roberto Campos
dc.creatorPereira, Helena de Souza
dc.creatorTeixeira, Valéria Laneuville
dc.date2017-12-21T16:04:33Z
dc.date2017-12-21T16:04:33Z
dc.date2016
dc.date.accessioned2023-09-26T23:56:21Z
dc.date.available2023-09-26T23:56:21Z
dc.identifierNOGUEIRA, Caio Cesar Richter; et al. Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera. Journal of Medicinal Plants Research, v.10, n.35, p.621-625, Sept. 2016.
dc.identifier1996-0875
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23745
dc.identifier10.5897/JMPR2016.6208
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895800
dc.descriptionFirst generation drugs such as zidovudine have been extensively used in clinical practice, resulting in the development of HIV resistance to these nucleoside analogs. Several studies have demonstrated the effective anti-HIV activity of natural products derived from seaweeds, suggesting promising sources of substances for the development of novel antiviral drugs. In this paper, the antiviral effect of extracts from the red seaweed Acanthophora spicifera on HIV-1 replication was evaluated in vitro. Peripheral blood mononuclear cells obtained using the Ficoll-Hypaque gradient were used for cytotoxicity and antiviral activity testing. The dichloromethane extracts, ethyl acetate, acetone, and methanol were found to have CC50 values of 31±7.4, 45±11, 38±3.5, and 179±25 μg/mL, respectively. With the control, the extract prepared in ethyl acetate inhibited approximately 60% of the viral load, which is the best result among the extracts. This same extract showed an IC50 value of 33.17±4.84 μg/mL for the reverse transcriptase. The EtOAc extract from A. spicifera showed to be an efficient HIV antiviral due to its phenolic compounds, as evaluated by nuclear magnetic resonance.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAcademic Journals
dc.rightsopen access
dc.subjectHIV-1
dc.subjectAcanthophora spicifera
dc.subjectAlga Marinha vermelha
dc.subjectAtividade antiviral
dc.subjectMarine natural products
dc.subjectred seaweed
dc.subjectAcanthophora spicifera
dc.subjectHIV-1
dc.subjectAntiviral activity
dc.subjectAnti-HIV-1 RT
dc.titleAnti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera
dc.typeArticle


Este ítem pertenece a la siguiente institución