Profiling Humoral Immune Response Against Pre-Erythrocytic and Erythrocytic Antigens of Malaria Parasites Among Neotropical Primates in the Brazilian Atlantic Forest

dc.creatorAssis, Gabriela Maira Pereira de
dc.creatorAlvarenga, Denise Anete Madureira de
dc.creatorPereira, Matheus de Oliveira Costa
dc.creatorSánchez-Arcila, Juan Camilo
dc.creatorCosta, Anielle de Pina
dc.creatorSouza Junior, Júlio César de Souza
dc.creatorNunes, Ana Julia Dutra
dc.creatorPissinatti, Alcides
dc.creatorMoreira, Silvia Bahadian
dc.creatorTorres, Leticia de Menezes
dc.creatorCosta, Helena Lott
dc.creatorTinoco, Herlandes da Penha
dc.creatorPereira, Valéria do Socorro
dc.creatorSoares, Irene da Silva
dc.creatorSouza, Taís Nóbrega de
dc.creatorNutmngia, Francis Babila
dc.creatorAdams, John H.
dc.creatorKano, Flora Satiko
dc.creatorHirano, Zelinda Maria Braga
dc.creatorDaniel-Ribeiro, Claudio Tadeu
dc.creatorFerreira, Joseli Oliveira
dc.creatorCarvalho, Luzia Helena
dc.creatorBrito, Cristiana Ferreira Alves de
dc.date2021-06-28T12:09:59Z
dc.date2021-06-28T12:09:59Z
dc.date2021
dc.date.accessioned2023-09-26T23:55:37Z
dc.date.available2023-09-26T23:55:37Z
dc.identifierASSIS, Gabriela Maria Pereira de et al. Profiling Humoral Immune Response Against Pre-Erythrocytic and Erythrocytic Antigens of Malaria Parasites Among Neotropical Primates in the Brazilian Atlantic Forest. Front. Cell. Infect. Microbiol., v. 11, Article 678996, 15 p, May 2021.
dc.identifier2235-2988
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/47889
dc.identifier10.3389/fcimb.2021.678996
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895673
dc.descriptionHuman malaria due to zoonotic transmission has been recorded in the Atlantic Forest, an extra-Amazonian area in Brazil, which are a challenge for malaria control. Naturally acquired humoral immune response against pre-erythrocytic and erythrocytic antigens of Neotropical primates (NP) was evaluated here to improve the knowledge about the exposure of those animals to the malaria transmission and support the identification of the potential reservoirs of the disease in the Atlantic Forest. Blood samples of 154 monkeys from three areas of the Atlantic Forest were used to identify IgG antibodies against peptides of the repeat region of the major pre-erythrocytic antigen, the circumsporozoite protein (CSP), of Plasmodium vivax (PvCSP), Plasmodium brasilianum/Plasmodium malariae (Pb/PmCSP), and Plasmodium falciparum (PfCSP) by ELISA. Antibodies against erythrocytic recombinant antigens of P. vivax, Apical membrane antigen 1 (PvAMA-1), Erythrocyte binding protein 2 (PvEBP-2) and domain II of Duffy binding protein (PvDBPII) were also evaluated. Parameters, such as age, sex, PCR positivity, Human malaria due to zoonotic transmission has been recorded in the Atlantic Forest, an extra-Amazonian area in Brazil, which are a challenge for malaria control. Naturally acquired humoral immune response against pre-erythrocytic and erythrocytic antigens of Neotropical primates (NP) was evaluated here to improve the knowledge about the exposure of those animals to the malaria transmission and support the identification of the potential reservoirs of the disease in the Atlantic Forest. Blood samples of 154 monkeys from three areas of the Atlantic Forest were used to identify IgG antibodies against peptides of the repeat region of the major pre-erythrocytic antigen, the circumsporozoite protein (CSP), of Plasmodium vivax (PvCSP), Plasmodium brasilianum/Plasmodium malariae (Pb/PmCSP), and Plasmodium falciparum (PfCSP) by ELISA. Antibodies against erythrocytic recombinant antigens of P. vivax, Apical membrane antigen 1 (PvAMA-1), Erythrocyte binding protein 2 (PvEBP-2) and domain II of Duffy binding protein (PvDBPII) were also evaluated. Parameters, such as age, sex, PCR positivity,Human malaria due to zoonotic transmission has been recorded in the Atlantic Forest, an extra-Amazonian area in Brazil, which are a challenge for malaria control. Naturally acquired humoral immune response against pre-erythrocytic and erythrocytic antigens of Neotropical primates (NP) was evaluated here to improve the knowledge about the exposure of those animals to the malaria transmission and support the identification of the potential reservoirs of the disease in the Atlantic Forest. Blood samples of 154 monkeys from three areas of the Atlantic Forest were used to identify IgG antibodies against peptides of the repeat region of the major pre-erythrocytic antigen, the circumsporozoite protein (CSP), of Plasmodium vivax (PvCSP), Plasmodium brasilianum/Plasmodium malariae (Pb/PmCSP), and Plasmodium falciparum (PfCSP) by ELISA. Antibodies against erythrocytic recombinant antigens of P. vivax, Apical membrane antigen 1 (PvAMA-1), Erythrocyte binding protein 2 (PvEBP-2) and domain II of Duffy binding protein (PvDBPII) were also evaluated. Parameters, such as age, sex, PCR positivity and captivity, potentially associated with humoral immune response were analyzed. Eighty-five percent of NP had antibodies against at least one CSP peptide, and 76% against at least one P. vivax erythrocytic antigen. A high percentage of adults compared to non-adults were seropositive and showed increased antibody levels. Neotropical primates with PCR positive for P. simium had a significantly higher frequency of positivity rate for immune response against PvEBP-2, PvDBPII and also higher antibody levels against PvDBPII, compared to PCR negative NPs for this species. Monkeys with PCR positive for P. brasilianum/P. malariae showed higher frequency of seropositivity and antibody levels against Pb/PmCSP. Levels of antibodies against Pb/PmCSP, PvEBP-2 and PvDBPII were higher in free-living than in captive monkeys from the same area. All Platyrrhine families showed antibodies against CSP peptides, however not all showed IgG against erythrocytic antigens. These findings showed a high prevalence of naturally acquired antibodies against CSP repeats in all studied areas, suggesting an intense exposure to infected-mosquitoes bites of NP from all families. However, mainly monkeys of Atelidae family showed antibodies against P. vivax erythrocytic antigens, suggesting blood infection, which might serve as potential reservoirs of malaria in the Atlantic Forest.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectMalária
dc.subjectImunidade Humoral
dc.subjectPrimatas neotropicais
dc.subjectMata Atlântica
dc.subjectAntígeno pré-eritrócitico
dc.subjectAntígenos eritróciticos
dc.subjectMalaria
dc.subjectHumoral response
dc.subjectNeotropical primates
dc.subjectAtlantic Forest
dc.subjectPre-erythrocytic antigen
dc.subjectErythrocytic antigens
dc.subjectImunidade Humoral
dc.titleProfiling Humoral Immune Response Against Pre-Erythrocytic and Erythrocytic Antigens of Malaria Parasites Among Neotropical Primates in the Brazilian Atlantic Forest
dc.typeArticle


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