RAB-5- and RAB-11-dependent vesicle-trafficking pathways are required for plasma membrane repair after attack by bacterial pore-forming toxin

dc.creatorLos, Ferdinand C. O.
dc.creatorKao, Cheng-Yuan
dc.creatorSmitham, Jane
dc.creatorMcDonald, Kent L.
dc.creatorHa, Christine
dc.creatorPeixoto, Christina A.
dc.creatorAroian, Raffi V.
dc.date2019-01-28T13:55:02Z
dc.date2019-01-28T13:55:02Z
dc.date2011
dc.date.accessioned2023-09-26T23:54:31Z
dc.date.available2023-09-26T23:54:31Z
dc.identifierLOS, Ferdinand C. O. et al. RAB-5- and RAB-11-Dependent Vesicle-Trafficking Pathways Are Required for Plasma Membrane Repair after Attack by Bacterial Pore-Forming Toxin. Cell Host & Microbe, v. 9, n. 2, p. 147–157, 17 fev. 2011.
dc.identifier1934-6069
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31325
dc.identifier10.1016/j.chom.2011.01.005
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895499
dc.descriptionNIH/NIGMS grant R01GM071603 and NIH/NIAID 2R01AI056189.
dc.descriptionPore-forming toxins (PFTs) secreted by pathogenic bacteria are the most common bacterial protein toxins and are important virulence factors for infection. PFTs punch holes in host cell plasma membranes, and although cells can counteract the resulting membrane damage, the underlying mechanisms at play remain unclear. Using Caenorhabditis elegans as a model, we demonstrate in vivo and in an intact epithelium that intestinal cells respond to PFTs by increasing levels of endocytosis, dependent upon RAB-5 and RAB-11, which are master regulators of endocytic and exocytic events. Furthermore, we find that RAB-5 and RAB-11 are required for protection against PFT and to restore integrity to the plasma membrane. One physical mechanism involved is the RAB-11-dependent expulsion of microvilli from the apical side of the intestinal epithelial cells. Specific vesicle-trafficking pathways thus protect cells against an attack by PFTs on plasma membrane integrity, via altered plasma membrane dynamics.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectAnimais
dc.subjectBactérias / metabolismo
dc.subjectFenômenos Fisiológicos Bacterianos
dc.subjectToxinas bacterianas / metabolismo
dc.subjectCaenorhabditis elegans / genética
dc.subjectCaenorhabditis elegans / metabolismo
dc.subjectCaenorhabditis elegans / microbiologia
dc.subjectProteínas Caenorhabditis elegans / genética
dc.subjectProteínas Caenorhabditis elegans / metabolismo
dc.subjectMembrana Celular / genética
dc.subjectMembrana Celular / metabolismo
dc.subjectMembrana Celular / microbiologia
dc.subjectVesículas citoplasmáticas / genética
dc.subjectVesículas citoplasmáticas / metabolismo
dc.subjectEndocitose
dc.subjectCélulas Epiteliais / metabolismo
dc.subjectCélulas Epiteliais / microbiologia
dc.subjectProteínas de Transporte Vesicular / genética
dc.subjectProteínas de Transporte Vesicular / metabolismo
dc.titleRAB-5- and RAB-11-dependent vesicle-trafficking pathways are required for plasma membrane repair after attack by bacterial pore-forming toxin
dc.typeArticle


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