Adipose-derived Mesenchymal Stromal Cells Modulate Lipid Metabolism and Lipid Droplet Biogenesis via AKT/mTOR -PPARγ Signalling in Macrophages

dc.creatorMoreira, Luciana Souza
dc.creatorSoares, Vinicius Cardoso
dc.creatorDias, Suelen da Silva Gomes
dc.creatorBozza, Patrícia T.
dc.date2020-04-19T20:35:28Z
dc.date2020-04-19T20:35:28Z
dc.date2019
dc.date.accessioned2023-09-26T23:53:49Z
dc.date.available2023-09-26T23:53:49Z
dc.identifierMOREIRA, Luciana Souza et al. Adipose-derived Mesenchymal Stromal Cells Modulate Lipid Metabolism and Lipid Droplet Biogenesis via AKT/mTOR –PPARγ Signalling in Macrophages. Scientific Reports, v. 9, 20304, p. 1-11, 2019.
dc.identifier2045-2322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/40881
dc.identifier10.1038/s41598-019-56835-8
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895375
dc.descriptionMesenchymal stromal cells (MSCs) are a potential therapy for many chronic inflammatory diseases due to their regenerative, immunologic and anti-inflammatory properties. The two-way dialogue between MSCs and macrophages is crucial to tissue regeneration and repair. Previous research demonstrated that murine adipose-derived MSC conditioned medium (ASCcm) reprograms macrophages to an M2-like phenotype which protects from experimental colitis and sepsis. Here, our focus was to determine the molecular mechanism of lipid droplet biogenesis in macrophages re-educated using ASCcm. Adipose-derived MSC conditioned medium promotes phosphorylation of AKT/mTOR pathway proteins in macrophages. Furthermore, increased expression of PPARγ, lipid droplet biogenesis and PGE2 synthesis were observed in M2-like phenotype macrophages (high expression of arginase 1 and elevated IL-10). Treatment with mTOR inhibitor rapamycin or PPARγ inhibitor GW9662 suppressed lipid droplets and PGE2 secretion. However, these inhibitors had no effect on arginase-1 expression. Rapamycin, but not GW9662, inhibit IL-10 secretion. In conclusion, we demonstrate major effects of ASCcm to reprogram macrophage immunometabolism through mTOR and PPARγ dependent and independent pathways.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNature Research
dc.rightsopen access
dc.subjectGotículas lipídicas
dc.subjectMacrófagos
dc.subjectMesenquimato derivado do adiposo Células estromais
dc.subjectMetabolismo
dc.subjectBiogênese
dc.subjectLipid Droplet Biogenesis
dc.subjectMacrophages
dc.subjectMesenchymal Stromal Cells
dc.subjectLipid Metabolism
dc.titleAdipose-derived Mesenchymal Stromal Cells Modulate Lipid Metabolism and Lipid Droplet Biogenesis via AKT/mTOR -PPARγ Signalling in Macrophages
dc.typeArticle


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