Determination of acetonitrile and cyanide in rat blood: Application to an experimental study

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMateus, Fabiano Henrique
dc.creatorLepera, José Salvador
dc.creatorLanchote, Vera Lucia
dc.date2014-05-27T11:21:17Z
dc.date2016-10-25T18:20:35Z
dc.date2014-05-27T11:21:17Z
dc.date2016-10-25T18:20:35Z
dc.date2005-03-01
dc.date.accessioned2017-04-06T01:12:42Z
dc.date.available2017-04-06T01:12:42Z
dc.identifierJournal of Analytical Toxicology, v. 29, n. 2, p. 105-109, 2005.
dc.identifier0146-4760
dc.identifierhttp://hdl.handle.net/11449/68162
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68162
dc.identifierWOS:000227342900004
dc.identifier2-s2.0-15244348958
dc.identifierhttp://jat.oxfordjournals.org/content/29/2/105.long
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889536
dc.descriptionMethods were developed for the analysis of acetonitrile and its metabolite cyanide in the blood of rats exposed to acetonitrile. Acetonitrile was analyzed by the headspace technique coupled to gas chromatography with detection by flame ionization, and cyanide was analyzed by high-performance liquid chromatography with fluorescence detection (λ ex = 418 nm and λ em = 460 nm) after derivatization of the ion with naphthalene 2,3-dicarboxyaldehyde and taurine. The quantitation limits of the methods for the analysis of acetonitrile and cyanide were 4.875 μg/mL and 0.025 μg/mL, respectively. The coefficients of variation of 10% or less obtained for intra- and interassay precision indicate the precision of these analytical methods and the systematic errors, all less than 5%, indicate that the methods are quite accurate. The methods were applied to an experimental study after the animals received acetonitrile at the doses of 2 mmol/kg or 5 mmol/kg.
dc.languageeng
dc.relationJournal of Analytical Toxicology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject2,3 naphthalenedicarboxaldehyde
dc.subjectacetonitrile
dc.subjectcyanide
dc.subjection
dc.subjecttaurine
dc.subjectaccuracy
dc.subjectanalytic method
dc.subjectanimal experiment
dc.subjectblood examination
dc.subjectcontrolled study
dc.subjectderivatization
dc.subjectfluorescence
dc.subjectgas chromatography
dc.subjecthigh performance liquid chromatography
dc.subjectmale
dc.subjectnonhuman
dc.subjectrat
dc.subjectAcetonitriles
dc.subjectAnimals
dc.subjectChromatography, High Pressure Liquid
dc.subjectCyanides
dc.subjectFlame Ionization
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReproducibility of Results
dc.titleDetermination of acetonitrile and cyanide in rat blood: Application to an experimental study
dc.typeOtro


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