An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway

dc.creatorSerpeloni, Mariana
dc.creatorMoraes, Carolina Borsoi
dc.creatorMuniz, João Renato Carvalho
dc.creatorMotta, Maria Cristina Machado
dc.creatorRamos, Augusto Savio Peixoto
dc.creatorKessler, Rafael Luis
dc.creatorInoue, Alexandre Haruo
dc.creatorRocha, Wanderson Duarte da
dc.creatorYamada-Ogatta, Sueli Fumie
dc.creatorFragoso, Stenio Perdigão
dc.creatorGoldenberg, Samuel
dc.creatorFreitas-Junior, Lucio Holanda
dc.creatorÁvila, Andréa Rodrigues
dc.date2018-11-14T17:05:51Z
dc.date2018-11-14T17:05:51Z
dc.date2011
dc.date.accessioned2023-09-26T23:53:45Z
dc.date.available2023-09-26T23:53:45Z
dc.identifierSERPELONI, Mariana et al. An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway. PLoS ONE, v. 6, n. 6, p. 1-14, 2011.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30033
dc.identifier10.1371/journal.pone.0020730
dc.identifier1932-6203
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895363
dc.descriptionIn eukaryotic cells, different RNA species are exported from the nucleus via specialized pathways. The mRNA export machinery is highly integrated with mRNA processing, and includes a different set of nuclear transport adaptors as well as other mRNA binding proteins, RNA helicases, and NPC-associated proteins. The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas disease, a widespread and neglected human disease which is endemic to Latin America. Gene expression in Trypanosoma has unique characteristics, such as constitutive polycistronic transcription of protein-encoding genes and mRNA processing by trans-splicing. In general, post-transcriptional events are the major points for regulation of gene expression in these parasites. However, the export pathway of mRNA from the nucleus is poorly understood. The present study investigated the function of TcSub2, which is a highly conserved protein ortholog to Sub2/ UAP56, a component of the Transcription/Export (TREX) multiprotein complex connecting transcription with mRNA export in yeast/human. Similar to its orthologs, TcSub2 is a nuclear protein, localized in dispersed foci all over the nuclei -except the fibrillar center of nucleolus- and at the interface between dense and non-dense chromatin areas, proposing the association of TcSub2 with transcription/processing sites. These findings were analyzed further by BrUTP incorporation assays and confirmed that TcSub2 is physically associated with active RNA polymerase II (RNA pol II), but not RNA polymerase I (RNA pol I) or Spliced Leader (SL) transcription, demonstrating participation particularly in nuclear mRNA metabolism in T. cruzi. The double knockout of the TcSub2 gene is lethal in T. cruzi, suggesting it has an essential function. Alternatively, RNA interference assays were performed in Trypanosoma brucei. It allowed demonstrating that besides being an essential protein, its knockdown causes mRNA accumulation in the nucleus and decrease of translation levels, reinforcing that Trypanosoma-Sub2 (Tryp-Sub2) is a component of mRNA transcription/export pathway in trypanosomes.
dc.formatapplication/pdf
dc.languagepor
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectRNA, Messenger
dc.subjectTranscription Genetic
dc.subjectGene Expression
dc.subjectARN Mensajero
dc.subjectTranscripción Genética
dc.subjectExpresión Génica
dc.subjectRNA Mensageiro
dc.subjectTrypanosoma cruzi
dc.subjectTranscrição Genética
dc.subjectTrypanosoma
dc.subjectExpressão Gênica
dc.titleAn essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway
dc.typeArticle


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