Six RNA viruses and forty-one hosts: viral small RNAs and modulation of small RNA repertoires in vertebrate and invertebrate systems

dc.creatorParameswaran, Poornima
dc.creatorSklan, Ella
dc.creatorWilkins, Courtney
dc.creatorBurgon, Trever
dc.creatorSamuel, Melanie A.
dc.creatorLu, Rui
dc.creatorAnsel, K. Mark
dc.creatorHeissmeyer, Vigo
dc.creatorEinav, Shirit
dc.creatorJackson, William
dc.creatorDoukas, Tammy
dc.creatorParanjape, Suman
dc.creatorPolacek, Charlotta
dc.creatorSantos, Flávia Barreto dos
dc.creatorJalili, Roxana
dc.creatorBabrzadeh, Farbod
dc.creatorGharizadeh, Baback
dc.creatorGrimm, Dirk
dc.creatorKay, Mark
dc.creatorKoike, Satoshi
dc.creatorSarnow, Peter
dc.creatorRonaghi, Mostafa
dc.creatorDing, Shou-Wei
dc.creatorHarris, Eva
dc.creatorChow, Marie
dc.creatorDiamond, Michael S.
dc.creatorKirkegaard, Karla
dc.creatorGlenn, Jeffrey S.
dc.creatorFire, Andrew Z.
dc.date2017-10-19T14:09:07Z
dc.date2017-10-19T14:09:07Z
dc.date2010
dc.date.accessioned2023-09-26T23:53:43Z
dc.date.available2023-09-26T23:53:43Z
dc.identifierPARAMESWARAN, Poornima; et al. Six RNA Viruses and Forty-One Hosts: Viral Small RNAs and Modulation of Small RNA Repertoires in Vertebrate and Invertebrate Systems. PLoS Pathog, v.6, n.2, e1000764., 21p, Feb. 2010.
dc.identifier1553-7366
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/22890
dc.identifier10.1371/journal.ppat.1000764
dc.identifier1553-7374
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895355
dc.descriptionWe have used multiplexed high-throughput sequencing to characterize changes in small RNA populations that occur during viral infection in animal cells. Small RNA-based mechanisms such as RNA interference (RNAi) have been shown in plant and invertebrate systems to play a key role in host responses to viral infection. Although homologs of the key RNAi effector pathways are present in mammalian cells, and can launch an RNAi-mediated degradation of experimentally targeted mRNAs, any role for such responses in mammalian host-virus interactions remains to be characterized. Six different viruses were examined in 41 experimentally susceptible and resistant host systems. We identified virus-derived small RNAs (vsRNAs) from all six viruses, with total abundance varying from "vanishingly rare" (less than 0.1% of cellular small RNA) to highly abundant (comparable to abundant micro-RNAs "miRNAs"). In addition to the appearance of vsRNAs during infection, we saw a number of specific changes in host miRNA profiles. For several infection models investigated in more detail, the RNAi and Interferon pathways modulated the abundance of vsRNAs. We also found evidence for populations of vsRNAs that exist as duplexed siRNAs with zero to three nucleotide 3' overhangs. Using populations of cells carrying a Hepatitis C replicon, we observed strand-selective loading of siRNAs onto Argonaute complexes. These experiments define vsRNAs as one possible component of the interplay between animal viruses and their hosts.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectVírus de RNA
dc.subjectSistemas vertebrados
dc.subjectSistemas invertebrados
dc.subjectHospedeiros
dc.subjectRepertórios de RNA pequenos
dc.subjectRNA viruses
dc.subjectVertebrate systems
dc.subjectInvertebrate Systems
dc.subjectForty-One Hosts
dc.subjectSmall RNA Repertoires
dc.titleSix RNA viruses and forty-one hosts: viral small RNAs and modulation of small RNA repertoires in vertebrate and invertebrate systems
dc.typeArticle


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