Desenvolvimento de Metodologia Analítica por Cromatografia em Fase Líquida de Alta Eficiência para Quantificação de Curcuminoides

Development of Analytical Methodology by High-Performance Liquid Chromatography to Curcuminoids Quantification

dc.creatorPaschoal, Ramon Gredilha
dc.creatorViçosa, Alessandra Lifsitch
dc.creatorSuarez-Fontes, Ana Mácia
dc.creatorVannier_Santos, Marcos André
dc.creatorMoreira, Davyson de Lima
dc.date2022-02-01T20:19:02Z
dc.date2022-02-01T20:19:02Z
dc.date2021
dc.date.accessioned2023-09-26T23:53:38Z
dc.date.available2023-09-26T23:53:38Z
dc.identifierPASCHOAL, Ramon Gredilha et al. Desenvolvimento de Metodologia Analítica por Cromatografia em Fase Líquida de Alta Eficiência para Quantificação de Curcuminoides. Revista Virtual de Química, v. 13, n. 5, p. 1181-1188, 2021.
dc.identifier1984-6835
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50980
dc.identifier10.21577/1984-6835.20210064
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895342
dc.descriptionTurmeric (Curcuma longa L.) is a species that has attracted great interest for containing bioactive curcuminoids that have some pharmacological and physiological activities. Among them, antimalarial, anti-inflammatory, antidepressant and anticancer effects that are mainly attributed to curcumin, demethoxycurcumin and bisdemethoxycurcumin stand out. This work aimed the development of an analytical methodology to optimization of the best conditions for sample preparation, chromatographic separation, detection, identification and quantification of curcumin, demethoxycurcumin and bisdemethoxycurcumin. The samples were weighed and solubilized to obtain a 200 μg/mL solution, analyzed in triplicate, using the following chromatographic parameters: flow rate at 1.2 mL/min; temperature at 50 oC; detection at 420 nm; total analysis time of 25 min and injection volume of 20 μL. The developed method showed good selectivity, resolution, signal symmetry and capacity factor for curcuminoids analysis. It is worth noting that the new method developed showed a scientific gain for being more efficient and with the change of column, there was a gain in selectivity and resolution for the separation of curcuminoids. In addition, the silica column modified with phenyl groups has a phase of medium polarity and has greater selectivity in the separation of aromatic compounds.
dc.formatapplication/pdf
dc.languagepor
dc.publisherSociedade Brasileira de Química
dc.rightsopen access
dc.subjectQuantificação de Curcuminoides
dc.subjectMetodologia Analítica
dc.subjectCromatografia
dc.subjectDesenvolvimento
dc.subjectFase Líquida de Alta Eficiência
dc.subjectCurcumin
dc.subjectDemethoxycurcumin
dc.subjectBisdemethoxycurcumin
dc.subjectAnalytical method
dc.subjectHPLC
dc.titleDesenvolvimento de Metodologia Analítica por Cromatografia em Fase Líquida de Alta Eficiência para Quantificação de Curcuminoides
dc.titleDevelopment of Analytical Methodology by High-Performance Liquid Chromatography to Curcuminoids Quantification
dc.typeArticle


Este ítem pertenece a la siguiente institución