Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation

dc.creatorValverde, Ludmila de Faro
dc.creatorPereira, Thiago de Almeida
dc.creatorDias, Rosane Borges
dc.creatorGuimarães, Vanessa Sousa Nazaré
dc.creatorRamos, Eduardo Antônio Gonçalves
dc.creatorSantos, Jean Nunes
dc.creatorGurgel Rocha, Clarissa Araújo
dc.date2017-02-13T13:01:00Z
dc.date2017-02-13T13:01:00Z
dc.date2016
dc.date.accessioned2023-09-26T23:53:35Z
dc.date.available2023-09-26T23:53:35Z
dc.identifierVALVERDE, L. F. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumor Biology, v.37, n.7, p. 9233–9241, 2016.
dc.identifier1010-4283
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/17798
dc.identifier10.1007/s13277-015-4763-6
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895336
dc.descriptionEthics approval and consent to participate The present research pro- posal received approval from our host institution’s review board. In- formed consent was obtained from all individual participants included in the study.
dc.descriptionFAPESB e CNPQ
dc.descriptionThe present study aimed to evaluate the role of Hedgehog (Hh) molecule expression in association with the clinical aspects of oral squamous cell carcinoma (OSCC), as well as angiogenesis and CD163+ macrophages. Twenty-eight cases of OSCC, nine cases of tumor-free resection margins (TM), and four cases of non-neoplastic oral mucosa (NNM) were submitted to immunohistochemistry to detect proteins Sonic Hedgehog (SHH), Indian Hedgehog (IHH), GLI1, CD163, and CD105. Protein colocalization with respect to SHH/CD163, IHH/CD163, GLI1/CD163, and GLI1/CD105 was assessed by immunohistochemical double staining. In tumor parenchyma, SHH and IHH were present in the cytoplasm of neoplastic cells, while GLI1 was observed in cytoplasm and nucleus. Endothelial cells were found to express SHH, IHH, and GLI1 within CD105+ vessels, and a positive correlation between infiltrating macrophage density (IMD) and microvascular density (MVD) was observed in cases of OSCC and TM. When compared to TM and NNM, the OSCC cases demonstrated higher immunoreactivity for SHH (p = 0.01), IHH (p = 0.39), GLI1 (p = 0.03), IMD (p = 0.0002), and MVD (p = 0.0002). Our results suggest the participation of the Hh pathway in OSCC by way of autocrine and paracrine signaling, in addition to the participation of both SHH and IHH ligands. Endothelial cells were also found to exhibit positivity with respect to Hh pathway components and we surmise that these molecules may play a role in tumor angiogenesis. CD163+ macrophages were also observed to express IHH, a ligand of this pathway, in addition to being associated with tumor neovascularization.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Nature
dc.rightsopen access
dc.subjectCâncer oral
dc.subjectProteínas Hedgehog
dc.subjectImuno-Histoquímica
dc.subjectOral cancer
dc.subjectSquamous cell carcinoma
dc.subjectHedgehog proteins
dc.subjectImmunohistochemistry
dc.subjectNeoplasias Bucais
dc.subjectProteínas Hedgehog
dc.subjectImuno-Histoquímica
dc.titleMacrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
dc.typeArticle


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