The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex

dc.creatorGodinho, Rodrigo Maciel da Costa
dc.creatorCrestani, Juliana
dc.creatorKmetzsch, Lívia
dc.creatorAraujo, Glauber de S.
dc.creatorFrases, Susana
dc.creatorStaats, Charley C.
dc.creatorSchrank, Augusto
dc.creatorVainstein, Marilene Henning
dc.creatorRodrigues, Marcio Lourenço
dc.date2019-03-07T19:09:52Z
dc.date2019-03-07T19:09:52Z
dc.date2014
dc.date.accessioned2023-09-26T23:52:24Z
dc.date.available2023-09-26T23:52:24Z
dc.identifierGODINHO, R. M. et al. The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex. Scientific Reports, v. 4, p. 1-11, 2014.
dc.identifier2045-2322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31990
dc.identifier10.1038/srep06198
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895129
dc.descriptionFungal pathogenesis requires a number of extracellularly released virulence factors. Recent studies demonstrating that most fungal extracellular molecules lack secretory tags suggest that unconventional secretion mechanisms and fungal virulence are strictly connected. Proteins of the endosomal sorting complex required for transport (ESCRT) have been recently associated with polysaccharide export in the yeast-like human pathogen Cryptococcus neoformans. Snf7 is a key ESCRT operator required for unconventional secretion in Eukaryotes. In this study we generated snf7Δ mutant strains of C. neoformans and its sibling species C. gattii. Lack of Snf7 resulted in important alterations in polysaccharide secretion, capsular formation and pigmentation. This phenotype culminated with loss of virulence in an intranasal model of murine infection in both species. Our data support the notion that Snf7 expression regulates virulence in C. neoformans and C. gattii by ablating polysaccharide and melanin traffic. These results are in agreement with the observation that unconventional secretion is essential for cryptococcal pathogenesis and strongly suggest the occurrence of still obscure mechanisms of exportation of non-protein molecules in Eukaryotes.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNature
dc.rightsopen access
dc.subjectVacuolar-sorting protein Snf7
dc.subjectVirulence determinants
dc.subjectCryptococcus neoformans complex
dc.titleThe vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex
dc.typeArticle


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