Reliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveys

dc.creatorBelitardo, Emilia M. M. Andrade
dc.creatorNery Jr, Nivison
dc.creatorTicona, Juan P. Aguilar
dc.creatorPortilho, Moyra Machado
dc.creatorMello, Iago O
dc.creatorRibeiro, Guilherme S.
dc.creatorReis, Mitermayer G.
dc.creatorCosta, Federico
dc.creatorCummings, Derek A. T.
dc.creatorKo, Albert I.
dc.creatorFofana, Mariam O.
dc.date2022-12-15T18:36:16Z
dc.date2022-12-15T18:36:16Z
dc.date2022
dc.date.accessioned2023-09-26T23:51:52Z
dc.date.available2023-09-26T23:51:52Z
dc.identifierBELITARDO, Emilia M. M. Andrade et al. Reliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveys. Diagnostic Microbiology and Infectious Disease, v. 104, n. 4, p. 1-12, 2022.
dc.identifier0732-8893
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/55983
dc.identifier10.1016/j.diagmicrobio.2022.115807
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895030
dc.descriptionNational Institutes of Health. UK Medical Research Council. Wellcome Trust. Coordenação Brasileira de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Bill & Melinda Gates Foundation. American Society of Tropical Medicine and Hygiene . Sendas Family Fund at the Yale School of Public Health.
dc.descriptionReliable and scalable seroepidemiology methods are needed to estimate SARS-CoV-2 incidence and monitor the dynamics of population-level immunity as the pandemic evolves. We aimed to evaluate the reliability of SARS-CoV-2 normalized ELISA optical density (nOD) at a single dilution compared to titers derived from serial dilutions. We conducted serial serosurveys within a community-based cohort in Salvador, Brazil. AntiS IgG ELISA (Euroimmun AG) was performed with 5 serial 3-fold dilutions of paired sera from 54 participants. Changes in nOD reliably predicted increases and decreases in titers (98.1% agreement, k = 95.8%). Fitting the relationship between nOD and interpolated titers to a log-log curve yields highly accurate predictions of titers (r2 = 0.995) and changes in titers (r2 = 0.975), using only 1 to 2 dilutions. This approach can significantly reduce the time, labor and resources needed for large-scale erosurveys to ascertain population-level changes in exposure and immunity .
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectSARS-CoV-2
dc.subjectSorologia
dc.subjectAnticorpos
dc.subjectDensidade ótica
dc.subjectTítulos
dc.subjectSARS-CoV-2
dc.subjectSerology
dc.subjectAntibody
dc.subjectOptical density
dc.subjectTiters
dc.subjectSARS-CoV-2
dc.subjectSorologia
dc.subjectAnticorpos
dc.titleReliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveys
dc.typeArticle


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