Reliability of point-of-care circulating cathodic antigen assay for diagnosing schistosomiasis mansoni in urine samples from an endemic area of Brazil after one year of storage at -20 degrees Celsius

dc.creatorFavre, Tereza Cristina
dc.creatorBeck, Lilian Christina Nóbrega Holsback
dc.creatorBezerra, Fernando Schemelzer Moraes
dc.creatorGraeff-Teixeira, Carlos
dc.creatorCoelho, Paulo Marcos Zech
dc.creatorEnk, Martin Johannes
dc.creatorKatz, Naftale
dc.creatorOliveira, Ricardo Riccio
dc.creatorReis, Mitermayer Galvão dos
dc.creatorPieri, Otávio Sarmento
dc.date2022-06-17T11:46:37Z
dc.date2022-06-17T11:46:37Z
dc.date2022
dc.date.accessioned2023-09-26T23:51:51Z
dc.date.available2023-09-26T23:51:51Z
dc.identifierFAVRE, Tereza Cristina et al. Reliability of point-of-care circulating cathodic antigen assay for diagnosing schistosomiasis mansoni in urine samples from an endemic area of Brazil after one year of storage at -20 degrees Celsius. Revista da Sociedade Brasileira de Medicina Tropical, v. 55, e0389-2021, p. 1 - 7, 2022.
dc.identifier1678-9849
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/53346
dc.identifier10.1590/0037-8682-0389-2021
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8895029
dc.descriptionBackground: The World Health Organization recommends reliable point-of-care (POC) diagnostic testing to eliminate schistosomiasis. Lateral flow immunoassay that detects schistosome circulating cathodic antigen (CCA) in urine to establish prevalence thresholds for intervention in endemic areas is recommended. Stored urine may be useful if surveying at-risk populations is delayed or interrupted by unforeseen circumstances, such as the current COVID-19 pandemic. This study evaluated the manufacturer’s claim that Schistosoma mansoni infection can be reliably diagnosed in urine samples stored at -20°C for one year. Methods: Two-hundred-forty-two subjects from an endemic site in Brazil provided one urine sample each for testing with URINE CCA (SCHISTO) ECO TESTE® (POC-ECO) and one stool sample each for testing with Kato-Katz (KK) and Helmintex® (HTX) as a robust reference standard for infection status. At least 2 ml of urine from each participant was stored at -20°C; after one year, 76 samples were randomly selected for POC-ECO retesting. Results: The POC-ECO agreement between freshly collected and stored urine was inadequate considering trace results as positive (Cohen’s kappa coefficient κ = 0.08) and negative (κ = 0.36). POC-ECO accuracy was not significantly greater than that of routine KK (54%; 95% confidence interval: 42.1%-65.5%). Conclusions: The precision and accuracy of POC-ECO have to be optimized in both freshly collected and stored urine before it can be recommended for use in control programs in Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedade Brasileira de Medicina Tropical
dc.rightsopen access
dc.subjectSchistosoma mansoni
dc.subjectTeste de ponto de atendimento (POC)
dc.subjectAntígeno Catódico Circulante (CCA); urina congelada
dc.subjectKato-Katz
dc.subjectHelmintex®
dc.subjectBrasil
dc.subjectSchistosoma mansoni
dc.subjectPoint-of-Care (POC) testing
dc.subjectCirculating Cathodic Antigen (CCA); frozen urine
dc.subjectKato-Katz
dc.subjectHelmintex®
dc.subjectBrazil
dc.titleReliability of point-of-care circulating cathodic antigen assay for diagnosing schistosomiasis mansoni in urine samples from an endemic area of Brazil after one year of storage at -20 degrees Celsius
dc.typeArticle


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