TcI, TcII and TcVI Trypanosoma cruzi samples from Chagas disease patients with distinct clinical forms and critical analysis of in vitro and in vivo behavior, response to treatment and infection evolution in murine model

dc.creatorOliveira, Maykon Tavares de
dc.creatorBranquinho, Renata Tupinambá
dc.creatorAlessio, Glaucia Diniz
dc.creatorMello, Carlos Geraldo Campos
dc.creatorPaiva, Nívia Carolina Nogueira de
dc.creatorCarneiro, Cláudia Martins
dc.creatorToledo, Max Jean de Ornelas
dc.creatorReis, Alexandre Barbosa
dc.creatorMartins Filho, Olindo Assis Martins
dc.creatorLana, Marta de
dc.date2018-06-21T13:06:03Z
dc.date2018-06-21T13:06:03Z
dc.date2017
dc.date.accessioned2023-09-26T23:51:33Z
dc.date.available2023-09-26T23:51:33Z
dc.identifierOLIVEIRA, Maykon Tavares de et al. TcI, TcII and TcVI Trypanosoma cruzi samples from Chagas disease patients with distinct clinical forms and critical analysis of in vitro and in vivo behavior, response to treatment and infection evolution in murine model. Acta Tropica, v. 167, p. 108-120, 2017
dc.identifier0001-706X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27038
dc.identifier10.1016/j.actatropica.2016.11.033
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894971
dc.descriptionThe clonal evolution of Trypanosoma cruzi sustains scientifically the hypothesis of association between parasite’s genetic, biological behavior and possibly the clinical aspects of Chagas disease in patients from whom they were isolated. This study intended to characterize a range of biological properties of TcI, TcII and TcVI T. cruzi samples in order to verify the existence of these associations. Several biological features were evaluated, including in vitro epimastigotegrowth, “Vero”cells infectivity and growth, along with in vivo studies of parasitemia, polymorphism of trypomastigotes, cardiac inflammation, fibrosis and response to treatment by nifurtimox during the acute and chronic murine infection. The global results showed that the in vitro essays (acellular and cellular cultures) TcII parasites showed higher values for all parameters (growth and infectivity) than TcVI, followed by TcI. In vivo TcII parasites were more virulent and originated from patients with severe disease. Two TcII isolates from patients with severe pathology were virulent in mice, while the isolate from a patient with the indeterminate form of the disease caused mild infection. The only TcVI sample, which displayed low values in all parameters evaluated, was also originated of an indeterminate case of Chagas disease. Response to nifurtimox was not associated to parasite genetic and biology, as well as to clinical aspects of human disease. Although few number of T. cruzi samples have been analyzed, a discreet correlation between parasite genetics, biological behavior in vitro and in vivo (murine model) and the clinical form of human disease from whom the samples were isolated was verified.
dc.description2024-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectTcI
dc.subjectTcII
dc.subjectTcVI DTUs
dc.subjectcaracteristicas biologicas
dc.subjectTrypanosoma cruzi
dc.subjectTcI
dc.subjectTcII
dc.subjectTcVI DTUs
dc.subjectBiological characteristics
dc.subjectDrug response
dc.subjectHuman disease
dc.subjectclinical forms
dc.titleTcI, TcII and TcVI Trypanosoma cruzi samples from Chagas disease patients with distinct clinical forms and critical analysis of in vitro and in vivo behavior, response to treatment and infection evolution in murine model
dc.typeArticle


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