Identification of B-Cell Linear Epitopes in the Nucleocapsid (N) Protein B-Cell Linear Epitopes Conserved among the Main SARS-CoV-2 Variants

dc.creatorSilva, Rodrigo N. Rodrigues da
dc.creatorConte, Fernando P.
dc.creatorSilva, Gustavo da
dc.creatorAlencar, Ana L. Carneiro
dc.creatorGomes, Paula R.
dc.creatorKuriyama, Sergio N.
dc.creatorNeto, Antonio A. F.
dc.creatorLima Jumior, Josué C. Lima
dc.date2023-04-13T12:02:46Z
dc.date2023-04-13T12:02:46Z
dc.date2023
dc.date.accessioned2023-09-26T23:51:31Z
dc.date.available2023-09-26T23:51:31Z
dc.identifierSILVA, Rodrigo N. Rodrigues da et al Identification of B-Cell Linear Epitopes in the Nucleocapsid (N) Protein B-Cell Linear Epitopes Conserved among the Main SARS-CoV-2 Variants. Viruses, v. 15, 923, p. 1 - 16, Apr. 2023.
dc.identifier1999-4915
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/57749
dc.identifier10.3390/v15040923
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894967
dc.descriptionAbstract: The Nucleocapsid (N) protein is highlighted as the main target for COVID-19 diagnosis by antigen detection due to its abundance in circulation early during infection. However, the effects of the described mutations in the N protein epitopes and the efficacy of antigen testing across SARS-CoV-2 variants remain controversial and poorly understood. Here, we used immunoinformatics to identify five epitopes in the SARS-CoV-2 N protein (N(34–48), N(89–104), N(185–197), N(277–287), and N(378–390)) and validate their reactivity against samples from COVID-19 convalescent patients. All identified epitopes are fully conserved in the main SARS-CoV-2 variants and highly conserved with SARS-CoV.Moreover, the epitopes N(185–197) and N(277–287) are highly conserved withMERS-CoV, while the epitopes N(34–48), N(89–104), N(277–287), and N(378–390) are lowly conserved with common cold coronaviruses (229E, NL63, OC43, HKU1). These data are in accordance with the observed conservation of amino acids recognized by the antibodies 7R98, 7N0R, and 7CR5, which are conserved in the SARS-CoV-2 variants, SARS-CoV andMERS-CoV but lowly conserved in common cold coronaviruses. Therefore, we support the antigen tests as a scalable solution for the population-level diagnosis of SARS-CoV-2, but we highlight the need to verify the cross-reactivity of these tests against the common cold coronaviruses.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectCOVID-19
dc.subjectImunoinformática
dc.subjectTeste de antígeno
dc.subjectVariantes do SARS-CoV-2
dc.subjectCOVID-1;
dc.subjectImmunoinformatic
dc.subjectAntigen test
dc.subjectSARS-CoV-2 variants
dc.titleIdentification of B-Cell Linear Epitopes in the Nucleocapsid (N) Protein B-Cell Linear Epitopes Conserved among the Main SARS-CoV-2 Variants
dc.typeArticle


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