A new reporter cell line for studies with proteasome inhibitors in Trypanosoma brucei

dc.creatorMoura, Danielle M. N.
dc.creatorMelo Neto, Osvaldo P. de
dc.creatorCarrington, Mark
dc.date2019-10-16T12:48:39Z
dc.date2019-10-16T12:48:39Z
dc.date2019
dc.date.accessioned2023-09-26T23:51:27Z
dc.date.available2023-09-26T23:51:27Z
dc.identifierMOURA, Danielle M. N.; MELO NETO, Osvaldo P. de; CARRINGTON, Mark. A new reporter cell line for studies with proteasome inhibitors in Trypanosoma brucei. Molecular and Biochemical Parasitology, v. 227, p. 15-18, 2019.
dc.identifier0166-6851
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36483
dc.identifier10.1016/j.molbiopara.2018.11.001
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894952
dc.descriptionA Trypanosoma brucei cell line is described that produces a visual readout of proteasome activity. The cell line contains an integrated transgene encoding an ubiquitin-green fluorescent protein (GFP) fusion polypeptide responsive to the addition of proteasome inhibitors. A modified version of T. brucei ubiquitin unable to be recognized by deubiquitinases (UbG76V) was fused to eGFP and constitutively expressed. The fusion protein is unstable but addition of the proteasome inhibitor lactacystin stabilizes it and leads to visually detectable GFP. This cell line can be widely used to monitor the efficiency of inhibitor treatment through detection of GFP accumulation in studies involving proteasome-mediated proteolysis, screening of proteasome inhibitors or other events related to the ubiquitin-proteasome pathway.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectLactacystin
dc.subjectProteasome
dc.subjectTrypanosoma brucei
dc.subjectUbiquitin
dc.subjectLinhagem Celular
dc.subjectAvaliação Pré-Clínica de Medicamentos / métodos
dc.subjectGenes Reporter
dc.subjectProteínas de Fluorescência Verde / genética
dc.subjectProteínas de Fluorescência Verde / metabolismo
dc.subjectHumanos
dc.subjectComplexo de Endopeptidases do Proteassoma / genética
dc.subjectComplexo de Endopeptidases do Proteassoma / metabolismo
dc.subjectInibidores de Proteassoma / farmacologia
dc.subjectTripanossomicidas / farmacologia
dc.subjectTrypanosoma brucei brucei / efeitos dos fármacos
dc.subjectTrypanosoma brucei brucei / enzimologia
dc.subjectTrypanosoma brucei brucei / genética
dc.subjectTripanossomíase Africana / parasitologia
dc.subjectUbiquitina / metabolismo
dc.titleA new reporter cell line for studies with proteasome inhibitors in Trypanosoma brucei
dc.typeArticle


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