Brasil | Article

Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.

dc.creatorMontalvão, Fabricio
dc.creatorAlmeida, Geisy Monteiro
dc.creatorSilva, Elisabeth Martins da
dc.creatorBorges, Valeria de Matos
dc.creatorVasconcellos, Rita de Cássia dos Santos
dc.creatorTakiya, Christina Maeda
dc.creatorLopes, Marcela de Freitas
dc.creatorNunes, Marise Pinheiro
dc.creatorDosReis, George Alexandre
dc.date2015-02-09T17:40:06Z
dc.date2015-02-09T17:40:06Z
dc.date2010
dc.date.accessioned2023-09-26T23:50:07Z
dc.date.available2023-09-26T23:50:07Z
dc.identifierMONTALVÃO, F. et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages. European Journal of Immunology, v. 40, n. 2, p. 417-425, 2010.
dc.identifier1521-4141
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/9464
dc.identifier10.1002/eji.200939606
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894727
dc.descriptionPhagocytic removal of apoptotic lymphocytes exacerbates replication of Trypanosoma cruzi in macrophages. We investigated the presence of Ab against apoptotic lymphocytes in T. cruzi infection and the role of these Ab in parasite replication. Both control and chagasic serum contained IgG Ab that opsonized apoptotic lymphocytes. Treatment of apoptotic lymphocytes with purified IgG from chagasic, but not control serum, reduced T. cruzi replication in macrophages. The protective effect of chagasic IgG depended on Fcgamma receptors, as demonstrated by the requirement for the intact Fc portion of IgG, and the effect could be abrogated by treating macrophages with an anti-CD16/CD32 Fab fragment. Chagasic IgG displayed increased reactivity against a subset of apoptotic cell Ag, as measured by flow cytometry and immunoblot analyses. Apoptotic lymphocytes treated with chagasic IgG, but not control IgG, increased production of TNF-alpha, while decreasing production of TGF-beta1 by infected macrophages. Increased control of parasite replication required TNF-alpha production. Previous immunization with apoptotic cells or injection of apoptotic cells opsonized with chagasic IgG reduced parasitemia in infected mice. These results indicate that Ab raised against apoptotic cells could play a protective role in control of T. cruzi replication by macrophages.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley-VCH Verlag GmbH & Co. KGaA
dc.rightsopen access
dc.subjectApoptosis
dc.subjectFc receptors
dc.subjectPhagocytosis
dc.subjectTrypanosoma cruzi
dc.subjectAnticorpos Antiprotozoários/imunologia
dc.subjectDoença de Chagas/imunologia
dc.subjectLinfócitos/imunologia
dc.subjectMacrófagos/imunologia
dc.subjectTrypanosoma cruzi/imunologia
dc.subjectFator de Necrose Tumoral alfa/secreção
dc.subjectTransferência Adotiva
dc.subjectAnimais
dc.subjectAnticorpos Antiprotozoários/farmacologia
dc.subjectApoptose
dc.subjectCélulas Cultivadas
dc.subjectDoença de Chagas/parasitologia
dc.subjectTécnicas de Cocultura
dc.subjectCitometria de Fluxo
dc.subjectImunoglobulina G/imunologia
dc.subjectLinfócitos/citologia
dc.subjectMacrófagos/citologia
dc.subjectMasculino
dc.subjectCamundongos
dc.subjectParasitemia/imunologia
dc.subjectFator de Crescimento Transformador beta1/metabolismo
dc.subjectTrypanosoma cruzi/efeitos de drogas
dc.titleApoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.
dc.typeArticle


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