| dc.creator | Brunoro, Giselle Villa Flor | |
| dc.creator | Carvalho, Paulo Costa | |
| dc.creator | Ferreira, André Teixeira da Silva | |
| dc.creator | Perales, Jonas | |
| dc.creator | Valente, Richard Hemmi | |
| dc.creator | Gallo, Claudia Vitória de Moura | |
| dc.creator | Pagnoncelli, Dante | |
| dc.creator | Neves-Ferreira, Ana Gisele da Costa | |
| dc.date | 2015-03-17T14:45:53Z | |
| dc.date | 2015-03-17T14:45:53Z | |
| dc.date | 2015 | |
| dc.date.accessioned | 2023-09-26T23:49:45Z | |
| dc.date.available | 2023-09-26T23:49:45Z | |
| dc.identifier | BRUNORO, Giselle Villa Flor. et al. Proteomic profiling of nipple aspirate fluid (NAF): exploring the complementarity of different peptide fractionation strategies. Journal of Proteomics., v. 117, p. 8 6–9 4, 2015. | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/9720 | |
| dc.identifier | 10.1016/j.jprot.2015.01.011 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8894659 | |
| dc.description | NAF is a breast fluid that is closely related to the tumor microenvironment and a valuable
sample for studying breast cancer. To performan in-depth proteomic analysis of this sample,
aliquots of a single NAF digest were analyzed by the following peptide-centric fractionation
strategies: a) 30-cm reversed-phase (RP) column on-line with an LTQ-Orbitrap XL; b) off-line
strong cation-exchange (SCX) column; and c) pI-based OFFGEL fractionation. All fractions from
approaches (b) and (c) were further analyzed on a 10-cm RP column hyphenated to the same
mass spectrometer. The RP-30 cm, SCX/RP-10 cm, and OFFGEL/RP-10 cm approaches identified
1676, 2930, and 3240 peptides, which corresponded to 193, 390 and 528 proteins,
respectively. In our cumulative dataset, 4466 distinct NAF peptides corresponded to a total of
557 proteins, of which only 34% were identified by all three approaches. No exclusive protein
identification was associated to the RP-30 cm approach, while SCX/RP-10 cm and OFFGEL/
RP-10 cm contributed to 28 and 166 exclusive identifications, respectively. Each approach
provided additional information related to energy metabolism (fermentation process/
carbohydrate biosynthesis). In conclusion, the pre-fractionation platforms used were
complementary for the comprehensive characterization of NAF and our work provides
methodological information for future quantitative cancer-related NAF sample studies. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Elsevier | |
| dc.rights | restricted access | |
| dc.subject | Pre-Fractionation | |
| dc.subject | Shotgun | |
| dc.subject | NAF | |
| dc.subject | Breast Fluid | |
| dc.subject | Armas de Fogo | |
| dc.subject | Mama | |
| dc.title | Proteomic profiling of nipple aspirate fluid (NAF): exploring the complementarity of different peptide fractionation strategies | |
| dc.type | Article | |
